文章摘要
Jingjun Sun,Jin Zhu,Zhenning Qiu,Yuhua Li,Guipeng Ding,Yi Zhu,Zhenqing Feng,Xiaohong Guan.[J].南京医科大学学报,2005,19(3):
The effect of MAGE-A1 gene on NIH3T3 cells
  
DOI:10.7655
中文关键词: 
英文关键词: melanoma antigen genes-A1  cell cycle  apoptosis  gene transfer  mobile ability
基金项目:
Jingjun Sun  Jin Zhu  Zhenning Qiu  Yuhua Li  Guipeng Ding  Yi Zhu  Zhenqing Feng  Xiaohong Guan
Department of Pathology, Nanjing Medical University, Nanjing 210029, China;Institute of Molecular Biology, Nanjing Medical University, Nanjing 210029, China;Institute of Molecular Biology, Nanjing Medical University, Nanjing 210029, China;Department of Pathology, Nanjing Medical University, Nanjing 210029, China;Department of Pathology, Nanjing Medical University, Nanjing 210029, China;Department of Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;Department of Pathology, Nanjing Medical University, Nanjing 210029, China;Institute of Molecular Biology, Nanjing Medical University, Nanjing 210029, China
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中文摘要:
      
英文摘要:
      Objective: Melanoma antigen genes(MAGE) genes have been found in many kinds of tumor tissue, but not in normal tissue except testis and placentas. The Ags encoded by MAGE genes therefore are strictly tumor-specific. The most current researches associated with these genes focus on the tumor vaccination using these Ags. Few reports are concerning these genes' functions. In this study, we investigated the role of MAGE-A1 gene on NIH3T3 cells after transferring with it. Methods: Clone the MAGE-A1 into the plasmids pEGFP-C3 and pcDNA3.1, then transfer the reconstructed plasmids and primary plasmids into the NIH3T3 cells using a new transfer reagent FuGENE 6. Selecting the positively transferred cells by G418. Identified by RT-PCR, Western blot, Immunocytochemistry,Laser Scanning Confocal Microscope and Fluoroscope. The cells mobile ability was measured with Millicell-PCF. The cell cycle and apoptosis were measured with Flow Cytometry. Results: The apoptosis rate of NIH3T3 cells that transferred with control plasmid pcDNA3.1was 13.4% and the raitos that stay in S phase and G2-M phase were 5.68% and 1.04% respectively. The apoptosis rate of NIH3T3 cells that transferred with pcDNA3.1-A1 was 0.90% and the ratios that stayed in S phase and G2-M phase were 19.31% and 13.47% respectively. The apoptosis rate of the cells that transferred with control plasmid pEGFP-C3 was 1.87 %, a little higher than 1.47 % of those transferred with pEGFP-C3-A1. Conclusion: The MAGE-A1 gene may enhance the cell cycle, inhibit the apoptosis and raise the mobile ability of NIH3T3 cells.
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