文章摘要
Ruzhi Zhang,Wenyuan Zhu,Chen Wang.[J].南京医科大学学报,2005,19(3):
Studies on arsenic trioxide induced mice melanoma Cloundman S91 cell apoptosis
  
DOI:10.7655
中文关键词: 
英文关键词: arsenic trioxide  melanoma Cloundman S91  apoptosis
基金项目:
Ruzhi Zhang  Wenyuan Zhu  Chen Wang
Department of Dermatology. the First Affiliated Hospital of Nanjing Medical University. Nanjing 210029, China;Department of Dermatology. the First Affiliated Hospital of Nanjing Medical University. Nanjing 210029, China;Department of Dermatology. the Chin-Japa Friendly Hospital. Beijing 100029, China
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中文摘要:
      
英文摘要:
      Objective: To invesfgate the effects of various concentrations As2O3 on malignant melanoma. Methods: The viability of Cloundman melanoma S91 cells treated with As2O3 was measured by MTT [3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide] assay. The apoptosis was determined by transferase-mediated dUTP nick end labeling (TUNEL) detection, and the morphology of apoptotic cells was observed through transmission electron microscopy (TEM). The cells growth phase were analyzed by flow cytometry (FCM). Results: A time-and dose-dependent decrease in cell viability was induced in S91 cells after treatment with As2Os at the concentration of 1-5 μmol/L respectively. The TUNEL indices were 0.033 ± 0.018, 0.062 ± 0.012, 0.102 ± 0.016, 0.132 ± 0.031,and 0.162 ± 0.027 respectively, which were much higher compared with the control group (0.017 ± 0.004, P < 0.01). The flow cytometry showed that hypodiploid peak after treatment with 3 μmol/L and 5 μmol/L of As2O3 for 48 h were 9.99% and 17.59% respectively,which increased significantly compared with the control cells (3.05% P < 0.01, ). The apoptotic morphology observed by transmission electron microscope showed the chromatin became condensed and attached to the inner surface of nuclear membrane. Conclusion: Arsenic trioxide can induce melanoma Cloundman S91 cell apoptosis at the concentration of 1-5 μmol/L, which will provide enhanced benefit in melanoma therapy.
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