文章摘要
Maolin He,Anmin Chen.[J].南京医科大学学报,2005,19(3):
Construction and transfection of sense/antisense eukaryotic expression vectors for human cathepsin L gene
  
DOI:10.7655
中文关键词: 
英文关键词: cathepsin L  RT-PCR  antisense RNA  Western blot
基金项目:
Maolin He  Anmin Chen
Department of Orthopaedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, WuHan 430030, China;Department of Orthopaedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, WuHan 430030, China
摘要点击次数: 1026
全文下载次数: 123
中文摘要:
      
英文摘要:
      Objective: To obtain sense/antisense eukaryotic expression vectors for human cathepsin L gene, and study the biological effects on human osteosarcoma cell line MG-63 after transfection. Methods: Cathepsin L gene sense/antisense eukaryotic expression vectors were constructed with recombinant technology and transfected into the human osteosarcoma cell line MG-63. The expression of cathepsin L gene mRNA was examined with RT-PCR and the expression of cathepsin L was examined with Western blot. Results: The sense/antisense recombinant eukaryotic expression vectors for cathepsin L were successfully constructed and transfected into MG-63 cell.Conclusion: Antisense cathepsin L gene can significantly inhibit the expression of cathepsin L mRNA and protein.
查看全文   查看/发表评论  下载PDF阅读器
关闭