文章摘要
Shenghua Wu,Chao LU,Ling Dong,Ziqing Chen.[J].南京医科大学学报,2005,19(5):
Jak1/STAT3 pathway mediates the inhibition of lipoxin A4 on TNF-α-induced DNA synthesis of glomerular mesangial cells in rats
  
DOI:10.7655
中文关键词: 
英文关键词: lipoxin  tumor necrosis factor  DNA synthesis  cyclin  STAT  mesangial cell
基金项目:
Shenghua Wu  Chao LU  Ling Dong  Ziqing Chen
Department of Pediatrics,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China;Department of Pediatrics,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China;Central Laboratory,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China;Central Laboratory,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China
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中文摘要:
      
英文摘要:
      Objective: To examine whether lipoxin A4 (LXA4) has an inhibitory effect on tumor necrosis factor-α(TNF-α)-induced DNA synthesis of glomerular mesangial cells of rat, and explore the molecular mechanisms of LXA4 action. Methods: Glomerular mesangial cells of rat were cultured and preincubated with LXA4 at different concentrations, and then treated with TNF-α( 10 ng/ml). DNA synthesis was assessed by the incorporation of [3H]-thymidine in mesangial cells. Expression of cyclin E protein was determined by Western blotting analysis. Activities of signal transducers and activators of transcription-3 (STAT3) were analyzed by electrophoretic mobility shift assay (EMSA). Results: TNF-α-stimulated DNA synthesis of mesangial cells, upregulation of cyclin E protein and STAT3 activities were inhibited by LXA4 in a dose-dependent manner. Conclusion: TNF-α-induced DNA synthesis of mesangial cells can be inhibited by TXA4probably through the mechanism of Jak1/STAT3 pathway-dependent signal transduction.
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