| Wei SHEN,Shenggang SUN,Xuebing CAO.[J].南京医科大学学报,2005,19(5): |
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| Minocycline protects the apoptosis of PC12 cells induced by 1-methyl-4-phenylpyridinium |
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| DOI：10.7655 |
| 中文关键词: |
| 英文关键词: minocycline PC12 cell apoptosis parkinson disease 1-methyl-4-mhenylpyridinium |
| 基金项目: |
| Wei SHEN Shenggang SUN Xuebing CAO |
| Department of Neurology,the affiliated Union Hospital of Tongji Medical Collage,Huazhong University of Science and Technology,Wuhan 430022,China;Department of Neurology,the affiliated Union Hospital of Tongji Medical Collage,Huazhong University of Science and Technology,Wuhan 430022,China;Department of Neurology,the affiliated Union Hospital of Tongji Medical Collage,Huazhong University of Science and Technology,Wuhan 430022,China |
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| 中文摘要: |
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| 英文摘要: |
| Objective: To explore the protective effect of minocycline on the apoptosis of cellular parkinsonism models induced by MPP+ . Methods: Using PC12 cells as the apoptotic model of dopaminergic neurons, MC and MPP+ were added into the culture medium of PC12 cells, and using MTT to assay the cell viability and metabolic state; The cells apoptosis was assayed by electrophoresis method and using flow cytometry FACS to assay the apoptosis ratio. Results: Added the MPP+ to get the concentration of 10μmol/L, the cellular parkinsonism model of apoptosis had been prepared. The pre-treatment of MC (100 μmol/L) could significantly increase the PC12 cell viability. The apoptosis ratio of MC + MPP+ group was significantly lower than that of MPP+ group, but was still significantly higher than that of control group. Conclusion: MC may protect the cell apoptosis induced by MPP+ to some extent. |
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