文章摘要
Wei SHEN,Shenggang SUN,Xuebing CAO.[J].南京医科大学学报,2005,19(5):
Minocycline protects the apoptosis of PC12 cells induced by 1-methyl-4-phenylpyridinium
  
DOI:10.7655
中文关键词: 
英文关键词: minocycline  PC12 cell apoptosis  parkinson disease  1-methyl-4-mhenylpyridinium
基金项目:
Wei SHEN  Shenggang SUN  Xuebing CAO
Department of Neurology,the affiliated Union Hospital of Tongji Medical Collage,Huazhong University of Science and Technology,Wuhan 430022,China;Department of Neurology,the affiliated Union Hospital of Tongji Medical Collage,Huazhong University of Science and Technology,Wuhan 430022,China;Department of Neurology,the affiliated Union Hospital of Tongji Medical Collage,Huazhong University of Science and Technology,Wuhan 430022,China
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英文摘要:
      Objective: To explore the protective effect of minocycline on the apoptosis of cellular parkinsonism models induced by MPP+ . Methods: Using PC12 cells as the apoptotic model of dopaminergic neurons, MC and MPP+ were added into the culture medium of PC12 cells, and using MTT to assay the cell viability and metabolic state; The cells apoptosis was assayed by electrophoresis method and using flow cytometry FACS to assay the apoptosis ratio. Results: Added the MPP+ to get the concentration of 10μmol/L, the cellular parkinsonism model of apoptosis had been prepared. The pre-treatment of MC (100 μmol/L) could significantly increase the PC12 cell viability. The apoptosis ratio of MC + MPP+ group was significantly lower than that of MPP+ group, but was still significantly higher than that of control group. Conclusion: MC may protect the cell apoptosis induced by MPP+ to some extent.
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