文章摘要
Songmei Geng,Shengshun Tan.[J].南京医科大学学报,2006,20(2):
Constructing skin-equivalents using hair follicle stem cells
  
DOI:10.7655
中文关键词: 
英文关键词: hair follicle stem cell  skin-equivalents
基金项目:
Songmei Geng  Shengshun Tan
Department of Dermatology, the Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China;Department of Dermatology, the Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China
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中文摘要:
      
英文摘要:
      Objective: To establish the method of constructing skin-equivalents (SE) using hair follicle stem cells(HFSC).Methods: K19 positive cells derived from hair were cultivated using serum-free medium KGM and seeded on dermal equivalents (DE).After the culture between the air-liquid interface for 14 days, SE were harvested and used for evaluation. Results: K19 positive cells chosen as HFSC were located in bulge of out root sheet in hair follicle. Cultivated HFSC could build a fully developed, multi-layered epidermis on the basis of DE, resembling the skin structure. Conclusion: HFSC located in out root sheet can differentiate into keratinocyte in vitro and be used for SE construction.
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