文章摘要
Jia Zhao, Xiaoan Liu,Lijun Ling.[J].南京医科大学学报,2007,(1):32~35
Detection of micrometastases in bone marrow and sentinel lymph nodes of breast cancer patients
投稿时间:2006-03-22  
DOI:10.7655
中文关键词: 
英文关键词: breast cancer  micrometastases  bone marrow  sentinel lymph nodes  cytokeratin
基金项目:
作者单位
Jia Zhao Department of General Surgery the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China 
Xiaoan Liu  
Lijun Ling  
摘要点击次数: 909
全文下载次数: 993
中文摘要:
      
英文摘要:
      Objective: To study the sensitivity and clinical significance of HE-staining,IHC and RT-PCR in detecting breast cancer micrometastases in bone marrow and sentinel lymph nodes(SLNs). Methods:After general anesthesia, all patients underwent bone marrow puncture and sentinel lymph node biopsy(SLNB) by 1% isosulfan blue, and then HE-staining,IHC and RT-PCR were used to detect micrometastases. Results:Of 62 patients with breast cancer whose axillary lymph nodes showed negative HE-staining results, 15 cases presented with positive RT-PCR and 9 cases showed positive IHC results positive in bone marrow micrometastases detection. PT-PCR and IHC showed good uniformity(kappa=0.6945)and there was significant difference in detective rate between these two methods(χ2=4.1667,P = 0.0412). In SLN samples, 13 showed positive RT-PCR results, while 7 showed positive IHC results. PT-PCR and IHC showed good uniformity(kappa=0.6483)and significant difference was also found in detective rate between these two methods(χ2=4.1667,P = 0.0412). Both bone marrow and SLN samples were RT-PCR positive in 3 cases, which indicated that bone marrow micrometastases did not always accompany SLN micrometastases(χ2=0.067,P = 0.796). Conclusion: Even if no axillary lymph node involvement or distant metastases are present in routine preoperative examination, micrometastases can still be detected in bone marrow or SLNs. Because the bone marrow micrometastases and axillary node micrometastses are not present simultaneously, combination test of multiple indicators will detect micrometastases more accurately.
查看全文   查看/发表评论  下载PDF阅读器
关闭