文章摘要
Bo Wang, Yi Lu,Cheng’en Pan,Xiaogang Zhang,Hui Li,Kewei Meng,Zheng Wu.[J].南京医科大学学报,2007,(1):47~50
Response of the xenograft endothelium in the concordant xenotransplantation
投稿时间:2006-04-28  
DOI:10.7655
中文关键词: 
英文关键词: endothelium  xenotransplantation  cyclosporine
基金项目:
作者单位
Bo Wang Department of Hepatobiliary Surgery the First Hospital of Xi’an Jiaotong University Xi’an 710061 China  
Yi Lu  
Cheng’en Pan  
Xiaogang Zhang  
Hui Li  
Kewei Meng  
Zheng Wu  
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中文摘要:
      
英文摘要:
      Objective: To investigate the response of the xenograft endothelium in the concordant hamster to rat cardiac xenotransplantation and the mechanism of acute vascular rejection. Methods: The animals were divided into 5 groups randomly: control group, CsA group, splenectomy group, D0 splenectomy+CsA group and D3 splenectomy+CsA group. Hamster heart was heterotopicaly transplanted to rat abdominal cavity. The graft survival was monitored by palpation of the rat abdominal wall. The histological and ultrastructural changes of the xenogafts were investigated. NF-κB and P-selectin expression in the xenograft were detected. Heme Oxigenase-1 and Bcl-2 expression were also detected in the xenografts of different groups. Results: The mean survival time of the xenografts in control group, CsA group, splenectomy group, D0 splenectomy+CsA group and D3 splenectomy+CsA group was 3.4 ± 0.55, 3.8 ± 0.45, 6.4 ± 1.52, 30 and 7.4 ± 1.14 days. The rejected graft showed typical acute vascular rejection in control group, CsA group,splenectomy group and D3 splenectomy+CsA group. Endothelial cells of the rejected xenograft showed dramatic assembly of ribosomes and expansion of the rough endoplasmic reticulum. However, the endothelium of the long-term survived grafts in D0 splenectomy+CsA group showed normal architecture. NF-κB and P-selectin expression were detected in the rejected xenografts. HO-1 expression was observed in the long-term survived xenografts in D0 splenectomy+CsA group. Conclusion: The endothelial cells of the xenograft might be activated during the acute vascular rejection. Expression of HO-1 might inhibit the upregulation of NF-κB and adhesion molecular which decreases the activation of the endothelium of the graft.
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