DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:


Qualitative and quantitative analysis of Chinese herb fructus chaenomelis
Author:
Affiliation:

Fund Project:

This work was supported by Natural Science Foundation of Jiangsu College & University (05KJD360142).

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    Abstract:

    Objective: To establish reliable methods for evaluating the quality of Chinese herb fructus chaenomelis. Methods: Qualitative analysis by Thin layer chromatography(TLC) , reference substances were Chaenomeles speciosa(Sweet) Nakai and oleanolic acid,a mixed solvent of chloroform-methanol(40 ∶ 1) was employed as the mobile phase,color developing agent was 10% sulfuric acid-ethanol solution.In the system of high performance liquid chromatography(HPLC), a Prontosil Eurobond C18 column (250 mm×4.0 mm,5 μm ) was used,the mobile phase was composed of acetonitrile-methanol-0.4% ammonium acetate solution(55 ∶ 25 ∶ 20),the flow rate was 1.0 ml/min with UV detected at 210 nm, the column temperature was maintained at room temperature. Results: In the system of TLC, oleanolic acid was separated successfully. In HPLC, the linear ranges of oleanolic acid and ursolic acid were 5.89~13.73 μg (R=0.9990)and 6.84~15.96 μg (R=0.9990), respectively. The average recoveries of oleanolic acid and ursolic acid were 97.52%(RSD=2 .58%), 98.21%(RSD=2.23%), respectively. Conclusion: The established TLC method can easily distinguish Chinese herb fructus chaenomelis from other commonly used crude drugs of the same family .The HPLC method for determining oleanolic acid and ursolic acid is simple, reproducible, accurate and feasible. The methods reported in this paper can be used scientifically and effectively to evaluate the quality of Chinese herb fructus chaenomelis.

    参考文献
    相似文献
    引证文献
引用本文

Shuhu Du, Lina Chen, Kunfang Ma, Hongjian Ji.[J].南京医科大学学报(自然科学版),2007,(1):51-54

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2006-04-07
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期: