文章摘要
Weijie Wang, Xiaobang Hu,Donghui Zhang, Jianhua Jiao,Yan Sun, Lei Ma,Changliang Zhu.[J].南京医科大学学报,2007,(2):63~67
Cloning and sequence analysis of β-actin gene from Aedes albopictus (Diptera: Culicidae)
投稿时间:2007-01-04  
DOI:10.7655
中文关键词: 
英文关键词: Aedes albopictus  β-actin  clone  nucleotide sequence  phylogeny
基金项目:
作者单位
Weijie Wang Department of Pathogen Biology Jiangsu Province Key Laboratory of Modern Pathogen Biology Nanjing Medical University Nanjing 210029 China.  
Xiaobang Hu  
Donghui Zhang  
Jianhua Jiao  
Yan Sun  
Lei Ma  
Changliang Zhu  
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中文摘要:
      
英文摘要:
      Objective: To obtain the complete β-actin gene from Aedes albopictus. Methods: Total RNA was extracted from C6/36 cells. Degenerate primers were designed based on the β-actin sequences of An. gambiae, Ae. aegypti, Cx. pipiens pallens and D. melanogaster. By RT-PCR, the product was amplified, purified, cloned into the pGT vector and sequenced. The β-actin sequence was aligned and phylogenetically analyzed by the BLAST program and the CLUSTAL W program. Results: A sequence of 1132 bp including an open reading frame of 1131 bp was obtained(GenBank DQ657949). The deduced protein had 376 amino acids. Aligned to SWISS-PROT, it exhibited a high level of identity with β-actins from Anopheles, Drosophila and Culex at the amino acid sequence level. Phylogenetic analysis indicated that Ae. albopictus β-actin was much more homologous with invertebrate β-actin than with vertebrate β-actin. Conclusion: The gene may be used as the internal control in the experiments of Ae. albopictus.
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