文章摘要
Qingxian Li,Yuhua Liao,Huiling Zhang,Yanying Jiang,Yanfu Wang.[J].南京医科大学学报,2007,(2):82~85
Inhibitory effect and mechanism of chuanxiongzine on multiplication of VSMC
投稿时间:2006-11-29  
DOI:10.7655
中文关键词: 
英文关键词: chuanxiongzine  vascular smooth muscle cell  proliferating cell nuclear antigen  c-myc
基金项目:
作者单位
Qingxian Li Department of Cardiology Union Hospital Tong Ji Medical College Hua Zhong University of Science & Technology Wuhan 430022 China 
Yuhua Liao Department of Cardiology Union Hospital Tong Ji Medical College Hua Zhong University of Science & Technology Wuhan 430022 China 
Huiling Zhang Department of Cardiology Affiliated Hospital Jining Medical College Jining272029 China  
Yanying Jiang Department of Ophthalmology Affiliated Hospital Jining Medical College Jining 272029 China 
Yanfu Wang Department of Cardiology Union Hospital Tong Ji Medical College Hua Zhong University of Science & Technology Wuhan 430022 China 
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中文摘要:
      
英文摘要:
      Objective: To study the inhibitory effect of chuanxiongzine on vascular smooth muscle cell (VSMC) proliferation and explore its molecular biology basis. Methods: we selected the VSMC cultured 4~8 generation from rat aorta thoracalis as research object. The objects were divided into four groups(Ⅰ)control group,(Ⅱ)chuanxiongzine(50 μg/ml)group,(Ⅲ)chuanxiongzine (100 μg/ml) group and(Ⅳ) chuanxiongzine(200 μg/ml) group. The inhibitory effect of chuanxiongzine on VSMC proliferation was investigated by cell counting, MTT and 3H-TdR incorporation assay. In order to illuminate the molecular biology mechanism of chuanxiongzine inhibiting VSMCs proliferation, the expression of proliferating cell nuclear antigen(PCNA) and C-myc were detected. Results: Chuanxiongzine could inhibit the proliferation of VSMC significantly in a dose- and time-dependent manner, compared with control group (P < 0.05). The expression of PCNA and c-myc were inhibited obviously and correlated with the concentration of chuanxiongzine (P < 0.05). Conclusion: Chuanxiongzine may play a considerable role in VSMC proliferation process. The inhibitory effect of chuanxiongzine in a dose- and time-dependent manner can be realized via down regulating the expression of PCNA and c-myc. In this study, The great theoretical fundament about Chinese medicine, which is used to treat atherosclerosis(AS), has been obtained.
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