文章摘要
Jiancang Ma,Qiang Li,Jun Zhao,Ying Guo,Qinghua Su,Zongzheng Ji.[J].南京医科大学学报,2007,(2):94~98
Effects of apigenin on cell proliferation of human pancreatic carcinoma cell line BxPC-3 in vitro
投稿时间:2006-10-31  
DOI:10.7655
中文关键词: 
英文关键词: apigenin  pancreatic carcinoma  cell cycle  gene
基金项目:
作者单位
Jiancang Ma Department of General Surgery, the Second Affiliated Hospital, Medical School of Xi?蒺an Jiaotong University,Xi?蒺an 710004, China 
Qiang Li  
Jun Zhao  
Ying Guo  
Qinghua Su  
Zongzheng Ji  
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中文摘要:
      
英文摘要:
      Objective: To observe the effects of apigenin on cell proliferation of human pancreatic carcinoma cell line BxPC-3 in vitro. Methods:The inhibitive effects of apigenin at different concentrations(0 ?滋mol/L, 100 ?滋mol/L, 200 ?滋mol/L, and 400 ?滋mol/L) on human pancreatic carcinoma cell line BxPC-3 were detected by MTT assays, transmission electron microscope,agarose gel electrophoresis and flow cytometry. The immunohistochemistry was used to detect the expression of Bcl-2 and Bax gene. Results:Apigenin at different concentrations could inhibit the proliferation of human pancreatic carcinoma cell lines BxPC-3, and the inhibitive effect was dose-dependent. The cell cycle of pancreatic carcinoma cells was arrested at G2/M phase. The results of immunohistochemistry showed that the density of apigenin increased, and the expression of Bcl-2 gene was reduced gradually. At the same time the expression of Bax gene was enhanced. Conclusion:Apigenin could inhibit the proliferation of human pancreatic carcinoma cell lines BxPC-3 in vitro. The effect of apoptosis was accompanied with the expression of Bcl-2 decrease and Bax increase.
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