文章摘要
Jiahong Xue,Zuyi Yuan,Yue Wu,Yan Zhao,Zhaofei Wan.[J].南京医科大学学报,2008,28(2):75~80
High glucose decreases the expression of ATP-binding cassette transporter G1 in human vascular smooth muscle cells
投稿时间:2007-11-26  
DOI:10.7655
中文关键词: 
英文关键词: vascular smooth muscle cells(VSMCs)  ABC transporters  glucose  oxidative stress  NF-κB pathway
基金项目:
作者单位
Jiahong Xue Key Laboratory of Environment Related Disease of Ministry Education, Department of Cardiovascular Medicine, the First Affiliated Hospital, Medical School of Xi’an Jiao tong University, Xi’an 710061, Shaanxi Province, China 
Zuyi Yuan  
Yue Wu  
Yan Zhao  
Zhaofei Wan  
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中文摘要:
      
英文摘要:
      Objective:ATP-binding cassette transporters(ABC) A1 and G1 play an important role in mediating cholesterol efflux and preventing macrophage foam cell formation. In this study, we examined the regulation of ABC transporters by high glucose in human vascular smooth muscle cells(VSMCs), the other precursor of foam cells. Methods:Incubation of human VSMCs with D-glucose(5 to 30 mM) for 1 to 7 days in the presence or absence of antioxidant and nuclear factor(NF)-κB inhibitors, the expressions of ABCA1 and ABCG1 were analyzed by real time PCR and Western blotting. Results:High glucose decreased ABCG1 mRNA and protein expression in cultured VSMCs, whereas the expression of ABCA1 was not significantly decreased. Down-regulation of ABCG1 mRNA expression by high glucose was abolished by antioxidant N-acetyl-L-cysteine(NAC) and NF-κB inhibitors, BAY 11-7085 and tosyl-phenylalanine chloromethyl-ketone(TPCK). Conclusion:High glucose suppresses the expression of ABCG1 in VSMCs, which is the possible mechanism of VSMC derived foam cell transformation.
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