文章摘要
Xiaoqin Jia,Yuefang Liu,Kaikun Liu,Qi Tang,Zhenqing Feng,Jianping Zhang.[J].南京医科大学学报,2008,28(5):286~289
Using SELDI-TOF-MS technology for screening serum markers of hepatic carcinoma in rats
投稿时间:2008-07-21  
DOI:10.7655
中文关键词: 
英文关键词: hepatic carcinoma model  SEIDI-TOF-MS  serum marker
基金项目:
作者单位
Xiaoqin Jia Key Laboratory of Antibody Technique of Ministry of Health, Nanjing 210029,Jiangsu Province, China 
Yuefang Liu Key Laboratory of Antibody Technique of Ministry of Health, Nanjing 210029,Jiangsu Province, China 
Kaikun Liu Department of General Surgery, the Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, Jiangsu Province, China 
Qi Tang Key Laboratory of Antibody Technique of Ministry of Health, Nanjing 210029,Jiangsu Province, China 
Zhenqing Feng Key Laboratory of Antibody Technique of Ministry of Health, Nanjing 210029,Jiangsu Province, China 
Jianping Zhang Department of General Surgery, the Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, Jiangsu Province, China 
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中文摘要:
      
英文摘要:
      Objective: To identify potential serum markers of hepatic carcinoma in rats through Surface-Enhanced Laser Desorption Ioniza-tion-Time of Flight-Mass Spectrometry(SELDI-TOF-MS) Technology. Methods: A rat model of hepatic carcinoma was established. The serum samples of hepatic carcinoma and normal rats were analyzed via SELDI-TOF-MS Technology. The changes of the serum protein fingerprint patterns were observed between the experimental group of hepatic carcinoma and the controls. The analysis was conducted by statistical software-Biomarker Wizard. Results: Fifty-six protein peaks in the serums were found. Within m/z 0-20 000, the protein peaks of m/z 1158, 8 835 and 15 302 of hepatic carcinoma serums were obviously higher in the rat models compared with those in the controls(P < 0.01). Conclusion: Three peaks were considered as potential biomarkers according to the serum protein fingerprint patterns of the hepatic carcinoma group and the control group.
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