文章摘要
Yu Zhang,Songwen Ju,Yongqian Shu.[J].南京医科大学学报,2009,29(1):10~14
Research on the effects of CD137 signaling on the function of CD3-CD56+NK cells
投稿时间:2008-10-29  
DOI:10.7655
中文关键词: 
英文关键词: CD137  CD3-CD56+NK cells  signal
基金项目:
作者单位
Yu Zhang Cancer Biotherapy Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China 
Songwen Ju  
Yongqian Shu  
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中文摘要:
      
英文摘要:
      To investigate the effects of CD137 signaling on the regulation of CD3-CD56+NK cells function. Methods: CD3-CD56+NK cells were treated with CD137 mAb or mouse IgG1 isotype control to study the effects of CD137 signaling on the function of CD3-CD56+NK cells. Cytotoxicity was measured by LDH activity in the supernatants of cell cultures; NKG2D and LFA-1 expression on CD3-CD56+NK cells were analyzed by flow cytometry. Results: CD137 was expressed on activated CD3-CD56+NK cells. The CD137 mAb enhanced the ability of CD3-CD56+NK cells to kill lung cancer cells(A549);Further studies revealed that the expression of NKG2D and LFA-1 was significantly increased in activated cells, and blockade of NKG2D and LFA-1 dramatically attenuated CD3-CD56+NK cytolysis of A549 cancer cells. Conclusion: CD137 signaling increases the ability of CD3-CD56+NK cells to kill cancer cells via up-regulating the expression of NKG2D and LFA-1.
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