文章摘要
Zhi Wang,Lili Zhang,Baogui Sun,Qiuyan Dai.[J].南京医科大学学报,2009,29(1):50~53
Induction of interleukin-8 production by angiotensin II in rat vascular smooth muscle cells
投稿时间:2008-10-13  
DOI:10.7655
中文关键词: 
英文关键词: Angiotensin II  Interleukin-8  Vascular smooth muscle cells
基金项目:
作者单位
Zhi Wang Department of Cardiology, the First People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080 , China 
Lili Zhang  
Baogui Sun  
Qiuyan Dai  
摘要点击次数: 1329
全文下载次数: 1350
中文摘要:
      
英文摘要:
      Objective: Interleukin-8 (IL-8) represents the prototypical chemokine that is made by a wide variety of cell types. Previously studies have suggested that angiotensin II (Ang II) is involved in atherogenesis through induction of proinflammatory cytokines such as interleukin-6 or monocyte chemoattractant protein-1 (MCP-1) in vascular smooth muscle cells (VSMCs), while the role of Ang II on IL-8 expression in VSMCs is poorly studied. Methods: In this study, VSMCs were isolated from the thoracic aorta of Sprague-Dawley rats. The expression of smooth muscle α-actin was confirmed by an immunocytochemical method. Semi-quantitative RT-PCR and enzyme-linked immunosorbent assay (ELISA) analyses were conducted to detect IL-8 expression. Results: In the present study we found that Ang II significantly increased the expression of IL-8 both at the mRNA and protein levels in rat VSMCs in a dose- and time-dependent manner. Conclusion: These findings suggested that Ang II may participate in atherosclerosis through induction of inflammatory mediator in VSMCs.
查看全文   查看/发表评论  下载PDF阅读器
关闭