文章摘要
Zongqiang Gao,Xiong Guo,Chen Duan,Weijuan Ma,Peng Xu,Ruiyu Liu,Qisheng Gu,Junchang Chen.[J].南京医科大学学报,2009,29(2):104~110
In vitro effects of sodium hyaluronate on the proliferation and the apoptosis in chondrocytes from patients with Kashin-Beck disease and osteoarthritis
投稿时间:2009-02-03  
DOI:10.7655
中文关键词: 
英文关键词: sodium hyaluronate  Kashin-Beck Disease(KBD)  osteoarthritis  chondrocyte  proliferation  apoptosis
基金项目:
作者单位
Zongqiang Gao Institute of Endemic Diseases, Faculty of Public Health, Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Medicine College of Xi’an Jiaotong University
Xi’an 710061, China 
Xiong Guo Institute of Endemic Diseases, Faculty of Public Health, Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Medicine College of Xi’an Jiaotong University
Xi’an 710061, China 
Chen Duan Institute of Endemic Diseases, Faculty of Public Health, Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Medicine College of Xi’an Jiaotong University
Xi’an 710061, China 
Weijuan Ma Institute of Endemic Diseases, Faculty of Public Health, Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Medicine College of Xi’an Jiaotong University
Xi’an 710061, China 
Peng Xu Institute of Endemic Diseases, Faculty of Public Health, Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Medicine College of Xi’an Jiaotong University
Xi’an 710061, China 
Ruiyu Liu Institute of Endemic Diseases, Faculty of Public Health, Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Medicine College of Xi’an Jiaotong University
Xi’an 710061, China 
Qisheng Gu Shanghai Qisheng Biological preparation Co.Ltd, Shanghai 201100, China 
Junchang Chen Department of Orthopedics Surgery, the Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China 
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中文摘要:
      
英文摘要:
      Objective:To identify the in vitro effects of sodium hyaluronate(HA) on the proliferation and the apoptosis of chondrocytes from patients with Kashin-Beck disease(KBD) and osteoarthritis(OA). Methods:Samples of articular cartilages from KBD and OA patients, as well as healthy volunteers(6 subjects in each of the 3 groups) were dissected, digested with collagenase and the cells cultured in monolayers. Chondrocytes from each sample were assigned to an untreated group and two HA-treated groups: H0(no HA), H100(HA, 0.1 g/L) and H500(HA, 0.5 g/L). The first passage chondrocytes were used to observe proliferation using the MTT assay, and apoptosis by flow cytometry through Annexin V/PI staining. Results:HA promoted proliferation of chondrocytes in all the three groups, and in KBD and OA groups, for cells cultured for 4 and 6 days, H500 significantly promoted the cell proliferation. The apoptotic rates of both KBD and OA group chondrocytes were in the order H500 < HA100 < H0. Conclusion:Sodium hyaluronate administration has a dose-dependent in vitro effect to promote proliferation and inhibit apoptosis of chondrocytes from patients with KBD and OA.
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