文章摘要
Zhijuan Cao,Xuan Xu,Linli Que,Qi Chen,Yuehua Li.[J].南京医科大学学报,2009,29(3):163~167
Dephosphorylation of cardiomyocyte Cx43 is associated with myocardial ischemia and reperfusion injury
投稿时间:2009-01-16  
DOI:10.7655
中文关键词: 
英文关键词: myocardium  I/R injury  connexin 43  inflammatory response
基金项目:
作者单位
Zhijuan Cao Department of Pathophysiology, Nanjing Medical University, Nanjing 210029, China 
Xuan Xu  
Linli Que  
Qi Chen  
Yuehua Li  
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中文摘要:
      
英文摘要:
      Objective:Myocardial ischemia/reperfusion(I/R) injury is the leading cause of death in the world. However, the details of the mecha-nism of its pathophysiology are still unknown. The present study was designed to investigate the role of connexin 43(Cx43) in acute models of myocardial I/R injury. Methods: Male C57BL/6 mice were subjected to myocardial ischemia(45 min) followed by reperfusion(4 hrs) in vivo. The whole operation was monitored using a two-lead ECG. Hearts were harvested and the level of protein was assessed by western blot analysis. Haematoxylin and Eosin(HE) staining was used to detect the extent of neutrophil infiltration. The expression level of IL-6 was detected by ELISA. Results: A murine myocardial I/R injury model was constructed successfully. Phosphorylated Cx43 decreased 83.45% while non-phosphorylated Cx43 increased 1.62- fold in the myocardium after I/R injury. Neutrophil infiltration and the expression of the inflammatory cytokine IL-6 increased in the myocardium following I/R. Conclusion: During myocardial I /R injury, cardiomyocyte Cx43 is dephosphorylated, and this may be associated with an inflammatory response.
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