文章摘要
Shanyong Yi,Kejun Nan,Aihua Yuan,Chuangxin Lu.[J].南京医科大学学报,2009,29(3):194~198
Isolation and characterization of cancer stem-like cells from MHCC97H Cell Lines
投稿时间:2008-12-29  
DOI:10.7655
中文关键词: 
英文关键词: hepatocellular carcinoma  CD133  cancer stem cell  tumorigenicity
基金项目:
作者单位
Shanyong Yi Cancer Center of the First Affiliated Hospital, College of Medicine of Xi’an Jiaotong University, Xi’an,710061, China 
Kejun Nan Cancer Center of the First Affiliated Hospital, College of Medicine of Xi’an Jiaotong University, Xi’an,710061, China 
Aihua Yuan Department of Cancer Center of the Nanjing First Hospital, Affiliated to Nanjing Medical University, Nanjing, 210006, China 
Chuangxin Lu Cancer Center of the First Affiliated Hospital, College of Medicine of Xi’an Jiaotong University, Xi’an,710061, China 
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中文摘要:
      
英文摘要:
      Objective:To identify and isolate CD133 positive cancer stem-like cells (CD133+ cells) from the highly invasive human hepatocellular carcinoma cell line(MHCC97H), and examine their potential for clonogenicity and tumorigenicity. Methods: CD133+ and CD133- cells were isolated from MHCC97H cell line by magnetic bead cell sorting(MACS), and the potentials of CD133+ cells for colony formation and tumorigenicity were evaluated by soft agar cloning and tumor formation following nude mice inoculation. Results:CD133+ cells represent a minority(0.5-2.0%) of the tumor cell population with a greater colony-forming efficiency and greater tumor production ability. The colony-forming efficiency of CD133+ cells in soft agar was significantly higher than CD133- cells(36.8±1.4 vs 12.9±0.8, P <0.05). After 6 weeks, 3/5 mice inoculated with 1×103 CD133+ cells, 4/5 with 1×104 CD133+ cells and 5/5 with 1×105 CD133+ cells developed detectable tumors at the injection site, while only one tumor was found in mice treated with same numbers of CD133- cells. Conclusion: CD133 may be a hallmark of liver cancer stem cells (CSC) in human hepatocellular carcinoma(HCC), because the CD133+ cells identified and isolated with anti-CD133 labeled magnetic beads from MHCC97H cell line exhibit high potentials for clonogenicity and tumorigenicity. These CD133+ cells might contribute to hepatocarcinogenesis, as well as the growth and recurrence of human HCC, and therefore may be a useful target for anti-cancer therapy.
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