文章摘要
Nan Yang,Yujie Sun,Changyan Ma.[J].南京医科大学学报,2009,29(4):236~240
Isolation and identification of proteins binding to the major breakpoint region(mbr) of bcl2 gene
投稿时间:2009-04-12  
DOI:10.7655
中文关键词: 
英文关键词: bcl2 gene  37 mbr  DNA-binding protein
基金项目:
作者单位
Nan Yang Key Laboratory of Human Functional Genomics of Jiangsu Province, Department of Cell Biology and Medical Genetics, Nanjing Medical University, Nanjing 210029, China 
Yujie Sun  
Changyan Ma  
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英文摘要:
      Objective: We have previously found that mbr is a regulatory element of the bcl2 gene. The objective of this study is to isolate and identify the proteins binding to the 37 mbr in the 3′-end of the mbr. Methods: Streptavidin magnetic particles were ligated to concatameric oligonucleotides of 37 mbr and incubated with the nuclear extracts of Jurkat cells. The DNA-binding proteins were eluted and then resolved by SDS-PAGE. After silver staining, the protein bands were excised and subjected to MALDI-TOF MS. Results: Several protein bands were detected after the isolation with magnetic particles, and Splicing factor, proline- and glutamine-rich(SFPQ), Poly(ADP-ribose) polymerase 1(PARP), and promyelocytic leukemia protein(PML) were identified by MALDI-TOF MS. Conclusion: Several proteins were isolated and identified from the 37 mbr-protein complex. Results of this study establish a foundation for further study of the mechanisms by which mbr executes its regulatory function.
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