文章摘要
Hui Qiang,Peiguo Gao,Chen Zhang,Zhibin Shi,Tao Wang,Lei Wang,Kunzheng Wang.[J].南京医科大学学报,2009,29(6):373~379
Effects of Panax notoginseng saponins on apoptosis induced by hydrogen peroxide in cultured rabbit bone marrow stromal cells via altering the oxidative stress level and down-regulating caspase-3
投稿时间:2009-07-16  
DOI:10.7655
中文关键词: 
英文关键词: Panax notoginseng saponins (PNS)  oxidative stress  bone marrow stromal cells (BMSCs)  apoptosis  caspase-3
基金项目:
作者单位
Hui Qiang Department of Orthopaedics, the Second Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710004, China 
Peiguo Gao Department of Orthopaedics, Hospital of Northwestern Polytechnical University, Xi’an 710072, China 
Chen Zhang Department of Orthopaedics, the Second Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710004, China 
Zhibin Shi Department of Orthopaedics, the Second Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710004, China 
Tao Wang Department of Orthopaedics, the Second Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710004, China 
Lei Wang Department of Orthopaedics, the Second Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710004, China 
Kunzheng Wang Department of Orthopaedics, the Second Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710004, China 
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中文摘要:
      
英文摘要:
      Objective: To investigate the effects of Panax notoginseng saponins (PNS) on hydrogen peroxide (H2O2)-induced apoptosis in cultured rabbit bone marrow stromal cells (BMSCs). Methods: The effects of different concentrations of PNS on proliferation and early osteoblast differentiation of BMSCs were determined by the MTT assay and an alkaline phosphatase (ALP) assay. An optimal effective concentration of PNS was determined and used in subsequent experiments. The cultured BMSCs were divided into three groups: untreated control, H2O2 treated, and PNS pretreatment of H2O2 treated. The oxidative stress level was assessed by superoxide dismutase (SOD) and malondialdehyde (MDA) assays. Flow cytometry was used to determine BMSC apoptosis by staining with annexinV-FITC/propidium iodide (PI). The activity of caspase-3 enzyme was measured by spectrofluorometry. Results: PNS (0.1g/L) significantly increased both BMSC proliferation rate and ALP activity, while it decreased the indicators of oxidative stress, caspase-3 activity, and the apoptosis rate of BMSCs induced by H2O2.. Conclusion: PNS, acting as a biological antioxidant, had a protective effect on H2O2-induced apoptosis in cultured rabbit BMSCs by decreasing oxidative stress and down-regulating caspase-3.
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