文章摘要
Rong Zhang,Meifen Xing,Weiwen Wang,Xiaofan Yang,Xiaohui Ji.[J].南京医科大学学报,2009,29(6):380~385
Effect of Interferon-alpha in systemic lupus erthematosus (SLE) serum on the differentiation and maturation of dendritic cells derived from CD34+ hematopoietic precursor cells
投稿时间:2009-07-30  
DOI:10.7655
中文关键词: 
英文关键词: lupus erythematosus, systemic  dendritic cells  interferon-alpha  serum
基金项目:
作者单位
Rong Zhang Laboratory Center for Basic Medical Sciences, Nanjing Medical University, Nanjing 210029, China 
Meifen Xing Department of Microbiology and Immunology, Nanjing Medical University, Nanjing 210029, China 
Weiwen Wang Clinical Laboratory, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing 210012, China 
Xiaofan Yang Department of Microbiology and Immunology, Nanjing Medical University, Nanjing 210029, China 
Xiaohui Ji Department of Microbiology and Immunology, Nanjing Medical University, Nanjing 210029, China 
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中文摘要:
      
英文摘要:
      Objective: To study the effect of interferon-alpha IFN-α in the serum of SLE patients on the differentiation and maturation of dendritic cells (DCs) derived from CD34+ hematopoietic precursor cells (HPCs). Methods: Serum samples from SLE patients and normal controls were collected and the concentration of IFN-α detected by ELISA. CD34+HPCs were purified from cord blood by a magnetic cell sorting system (MACS), and cultured to differentiate to DCs. Normal serum, normal serum with exogenous IFN-α, SLE serum with raised levels of IFN-α, or SLE serum with anti-IFN-α neutralizing antibody was added to the culture medium. The phenotype of DCs was analyzed by flow cytometry (FCM) and the capacity of DCs to stimulate allogenic T lymphocyte proliferation was evaluated in a mixed lymphocyte reaction by the Cell Counting Kit-8. Cytokine production was assessed by ELISA. Results: Serum levels of IFN-α were significantly higher in SLE patients than in normal controls and this correlated positively with disease activity. Cultured in SLE serum with raised levels of IFN-α, CD34+ HPCs could differentiate into DCs that expressed higher levels of HLA-DR, CD80 and CD86, and showed an enhanced allogenic T-cell stimulatory capacity, while producing lower levels of IL-12 and higher amounts of IL-10 compared with those DCs cultured in normal serum. Conclusion: Increased levels of IFN-α in SLE serum promotes the differentiation and maturation of DCs derived from CD34+ HPCs and could contribute to the pathogenesis of SLE.
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