Page 7 - 南京医科大学学报自然科学版

第42卷第10期 南京医科大学学报（自然科学版）
2022年10月 Journal of Nanjing Medical University（Natural Sciences） ·1349 ·

·基础医学·

维奈克拉联合地西他滨、阿柔比星对急性髓系白血病细胞增殖

和凋亡的影响

尹金玉，徐子瑶，孙 倩，刘文洁，洪 鸣，钱思轩 *

南京医科大学第一附属医院血液科，江苏 南京 210029

［摘 要］ 目的：观察维奈克拉（venetoclax，ABT⁃199）联合地西他滨（decitabine，DAC）、阿柔比星（aclarubicin，ACM）对急性髓
系白血病（acute myeloid leukemia，AML）细胞株HL60、KG⁃1细胞增殖和凋亡的影响。方法：CCK⁃8法检测不同浓度ABT⁃199、
DAC、ACM单药及ABT⁃199+DAC、ABT⁃199+ACM、ABT⁃199+ACM+DAC 3种药物联合方式对HL60、KG⁃1细胞增殖的影响，使用
Compusyn软件分析药物联合是否具有协同作用；流式细胞术检测上述单药及药物联合对HL60、KG⁃1细胞凋亡比例和细胞周
期分布的影响。结果：ABT⁃199、DAC、ACM单药均抑制HL60、KG⁃1细胞增殖。相比单药ABT⁃199，3种药物联合方式均显著增
强对 HL60、KG⁃1 细胞增殖的抑制作用，且均具有协同作用。ABT⁃199 联合用药明显促进 HL60、KG⁃1 细胞凋亡。DAC、ABT⁃
199+DAC、ABT⁃199+ACM+DAC可促使HL60、KG⁃1细胞周期阻滞于G2/S期。结论：ABT⁃199联合DAC、ACM抑制AML细胞增
殖且具有协同作用，可促进AML细胞凋亡和细胞周期阻滞。
［关键词］ 急性髓系白血病；维奈克拉；地西他滨；阿柔比星；细胞增殖；细胞凋亡；细胞周期；协同作用
［中图分类号］ R733.7 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）10⁃1349⁃08
doi：10.7655/NYDXBNS20221001

Influence of venetoclax combined with decitabine and/or aclarubicin on the proliferation
and apoptosis of acute myeloid leukemia cells
*
YIN Jinyu，XU Ziyao，SUN Qian，LIU Wenjie，HONG Ming，QIAN Sixuan
Department of Hematology，the First Affiliated Hospital of Nanjing Medical University，Nanjing 210029，China

［Abstract］ Objective：To investigate the effects of venetoclax（ABT⁃199）combined with decitabine（DAC）and/or aclarubicin（ACM）
on the proliferation and apoptosis of acute myeloid leukemia（AML）cell lines（HL60 and KG⁃1）. Methods：HL60 and KG⁃1 were
treated with different concentrations of ABT⁃199，DAC，ACM，ABT⁃199+DAC，ABT⁃199+ACM and ABT⁃199+ACM+DAC. The cell
proliferation was determined by CCK⁃8 method. Compusyn software was used to analyze whether the drug combinations had synergistic
effect. The apoptosis and cell cycle were detected by flow cytometry（FCM）. Results：ABT⁃199，DAC and ACM inhibited the growth of
HL60 and KG⁃1 respectively. The three ways of drug combinations significantly inhibited the cell proliferation of HL60 and KG⁃1，
compared with ABT⁃199 alone，and all had synergistic effect. The combinations of ABT⁃ 199 significantly induced the apoptosis of
HL60 and KG⁃1. The cell cycle was arrested at G2/S phase in HL60 and KG⁃1 cells treated by DAC，the combination of ABT⁃199 and
DAC，or the combination of ABT⁃199，ACM and DAC. Conclusion：ABT⁃199 combinations significantly inhibited the proliferation of
AML cell lines and had synergistic effect. ABT⁃199 combined with ACM and DAC promoted the apoptosis and arrested the cell cycle
of AML cells.
［Key words］ acute myeloid leukemia；venetoclax；decitabine；aclarubicin；cell proliferation；apoptosis；cell cycle；synergism
［J Nanjing Med Univ，2022，42（10）：1349⁃1356］

［基金项目］ 国家自然科学基金（81870119，82170153）
∗
通信作者（Corresponding author），E⁃mail：qiansx@medmail.com.cn