Objective:To evaluate the impact of preimplantation genetic diagnosis procedures on the heat stress response in the offspring using mouse model. Methods:We use the mouse models,which divided into PGD group and Normal group. At 5 weeks of age,evaluate the stress system of PGD offspring under 45℃ heat stress. Results:Under heat stress,the mean death time is 59.31 min and 79.63 min in PGD and Normal group, respectively(P < 0.001). There is no significant difference between the two groups of mice under the stress,such as serum hormones,structure and neuronal activity of locus coeruleus and hypothalamus. Similarly,there is no significant difference of adrenal structure between two groups,and the level of adrenaline,noradrenaline and corticosterone is the same trend. Conclusion:The different response ability between two groups under heat stress is not related to the function of locus coeruleus,hypothalamus and adrenal. It may be related to the further function of their secretioned hormones.