Objective:To investigate the expression level of long noncoding RNA BANCR in non-small cell lung cancer (NSCLC) tissues and cell lines,and to study the effect of BANCR on NSCLC cell proliferation and invasion. Methods:Quantitative PCR was performed to detect the relative expression of BANCR in NSCLC cell lines and tissues. pCDNA-BANCR was transfected into SPC-A1 cells and A549 cells to up-regulate BANCR expression. Transwell assays were performed to detect the effect of BANCR on NSCLC proliferation and invasion. Western blotting was used to detect the expression level of E-cadherin and Vimentin,markers of epithelial-mesenchymal transition. Results:This study showed that BANCR was lowly expressed both in NSCLC samples and cell lines compared with their corresponding normal tissues and cell lines. MTT assays indicated that up-regulated BANCR inhibited the proliferation and invasion of SPC-A1 and A549 cells. BANCR overexpression elevated the expression of E-cadherin and suppresed the level of Vimentin,thus influencing epithelial-mesenchymal transition. Conclusion:BANCR promotes NSCLC cell proliferation and invasion by affecting epithelial-mesenchymal transition.