miRNA-873 促进肝癌细胞的迁移与侵袭
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

江苏省六大人才高峰项目基金(NO2014-WSW-005)


MiRNA-873 promotes migration and invasion of hepatocellular carcinoma
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:检测miRNA-873在肝细胞肝癌组织以及细胞系中的表达情况,并进一步探讨miRNA-873对肝癌细胞迁移和侵袭的影响。方法:实时定量逆转录聚合酶链反应 (RT-PCR)检测肝细胞肝癌组织、癌旁组织以及细胞系中miRNA-873的表达水平。分别用划痕实验和Transwell 细胞侵袭实验检测细胞迁移和侵袭能力。蛋白质印迹(Western blot)检测Janus激酶2(Janus kinase 2,JAK2)和信号转导子与转录活化子3(signal transduction and transcriptional activation 3,STAT3)活性的变化。结果:肝癌组织和肝癌细胞系中 miRNA-873表达水平明显高于对应的癌旁组织和正常肝脏细胞。RT-PCR证实转染miRNA-873 inhibitor或mimics后miRNA-873的表达量明显降低或升高(P<0.01)。与对照组(NC)相比,敲低miRNA-873 明显抑制肝癌细胞HepG2迁移和侵袭,差异有统计学意义(P<0.05)。过表达miRNA-873 则明显促进肝癌细胞Huh7迁移和侵袭,差异有统计学意义(P<0.05)。miRNA-873可明显活化JAK2/STAT3信号通路。结论:miRNA-873在肝癌中高表达,且可能通过活化JAK2/STAT3信号通路促进肝癌的迁移与侵袭。

    Abstract:

    Objective: To investigate the expression of miRNA-873 in hepatocellular carcinoma(HCC) tissues and cell lines, further study the effect of miRNA-873 on migration and invasion in hepatocellular carcinoma cell lines. Methods: The expression of miRNA-873 in HCC tissues and cell lines was detected by RT-PCR. Wound-healing experiment and Transwell invasion were used to examine migration and invasion of cells, respectively. Western blot was used to detect the activity of Janus kinase 2(JAK2) and signal transduction and transcriptional activation 3(STAT3). Results: The results showed that miRNA-873 was upregulated in HCC tissues and HCC cell lines, compared with that in the normal control. RT-PCR showed that the expression of miRNA-873 decreased or increased after miRNA-873 inhibitor or mimic transfection, respectively(P<0.01). Compared with the normal control, knockdown of miR-873 significantly inhibited the migration and invasion of HepG2(P<0.05). However, overexpression of miRNA-873 had the opposite effect(P<0.05). We also found that miRNA-873 significantly activated JAK2/STAT3 signal pathway. Conclusion: MiRNA-873 was overexpressed in HCC and might promote HCC migration and invasion through JAK2/STAT3 signal pathway.

    参考文献
    相似文献
    引证文献
引用本文

韩国勇,张 龙,陈志强,王小微,朱 勤,黄新立,王学浩. miRNA-873 促进肝癌细胞的迁移与侵袭[J].南京医科大学学报(自然科学版),2017,(9):1076-1080

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2017-03-30
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2017-09-25
  • 出版日期:
通知关闭
郑重声明