Objective：This study aimed to study the factors influencing the human immunodeficiency virus（HIV）couple testing among men who have sex with men（MSM），to provide the basis for formulating HIV couple testing strategies for MSM population. Method：①A 1∶2 matching analysis was conducted among MSM who did couple testing identified at the survey on AIDS prevention and intervention in Nanjing from May to October in 2017，and the control group was among the people who did not do the couple testing in the same survey of the same period of time. Each case was matched by age（±5），sexual orientation and marital status. ②The questionnaire design was based on the information of demography and behavioristics，using network survey. ③The data was screened by Excel first，then the logistic regression model is used for univariate and multivariate analysis. Results：A total of 73 patients were tested for sexual partners，while 146 subjects were matched in the control group. Univariate analysis showed that the number of previous HIV tests（χ2=6.195，P＜0.05），the number of previous HIV self-testing（χ2=33.658，P＜0.05）and the sex role of “0”（χ2=5.223，P＜0.05）had statistical significance with the sexual partner test. Multivariate analysis showed that the number of HIV self-testing in the past（OR=8.502，95%CI：3.474，20.805）and the sex role of “0”（OR=0.397，95%CI：0.163，0.969）were statistically significant. Conclusion：The number of HIV self-testing in the past was the promoting factor of couple testing，and the sex role of “0” was the obstacle factor for the testing.