文章摘要
徐锦雯,赵丽萍,朱春华,韩 媛,张爱华,黄松明.热休克蛋白70-hom基因多态性与儿童过敏性紫癜的相关性[J].南京医科大学学报,2012,(8):1075~1078
热休克蛋白70-hom基因多态性与儿童过敏性紫癜的相关性
HSP70-hom polymorphism and susceptibility to childhood Henoch-Schonlein purpura
投稿时间:2012-03-30  
DOI:10.7655
中文关键词: 热休克蛋白70  过敏性紫癜  多态性
英文关键词: heat shock protein 70  Henoch-Schonlein purpura  polymorphism
基金项目:南京市医学重点发展项目(ZKX08007)
作者单位
徐锦雯 南京医科大学附属无锡人民医院儿科,江苏 无锡 214023 
赵丽萍 南京医科大学附属无锡人民医院儿科,江苏 无锡 214023 
朱春华 南京医科大学附属南京儿童医院儿肾内科,江苏 南京 210008 
韩 媛 南京医科大学附属南京儿童医院儿肾内科,江苏 南京 210008 
张爱华 南京医科大学附属南京儿童医院儿肾内科,江苏 南京 210008 
黄松明 南京医科大学附属南京儿童医院儿肾内科,江苏 南京 210008 
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中文摘要:
      目的:探讨热休克蛋白70-hom(heat shock protein 70-hom,HSP70-hom)基因多态性与儿童过敏性紫癜发病的相关性?方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测131例过敏性紫癜患儿和66例正常儿童的HSP70-hom基因的+2437位核苷酸的Nco Ⅰ限制性内切酶位点的多态性?结果:HSP70-hom各基因型频率在过敏性紫癜组中分别为1.5%(TT)?34.4%(TC)和64.1%(CC),在对照组中分别为19.7%(TT)?19.7%(TC)和60.6%(CC),两组之间基因型分布频率的差异具有统计学意义(χ2 = 22.318,P < 0.05);T和C等位基因频率在过敏性紫癜组中分别为18.7%(T)和81.3%(C),在对照组中为29.5%(T)和70.5%(C),差别亦具有统计学意义(χ2 = 5.949,P < 0.05)?与携带TT基因型者比较,携带至少1个C等位基因(即TC和CC基因型)的个体患过敏性紫癜的风险增加18.83倍(95%CI:3.95~89.75)?过敏性紫癜非肾脏损害组与肾损害组TT?TC?CC基因型频率分别为2.4%?32.1%?65.5%和0%?38.3%?61.7%,差异不具有统计学意义(χ2 = 0.379,P > 0.05)?结论:HSP70-hom +2437T/C基因多态性是儿童过敏性紫癜发病的遗传易感因素,但与肾脏损害发生无显著相关性?
英文摘要:
      Objective:To investigate the potential involvement of gene variants of heat shock protein 70-hom(HSP70-hom) in childhood Henoch-Schonlein purpura. Methods:Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to analyze the polymorphisms of HSP70-hom +2437T/C in 131 children with Henoch-Schonlein purpura and 66 health controls. Results:HSP70-hom gene frequencies in children with Henoch-Schonlein purpura were 1.5%(TT),34.4%(TC),64.1%(CC),and 19.7%(TT),19.7%(TC),60.6%(CC) in health controls,the differences between two groups were statistically significant. In addition,the T,C allele frequencies were 18.7%(T),81.3%(C) in Henoch-Schonlein purpura group,and were 29.5%(T),70.5%(C) in control groups,the differences were statistically significant,too. Children carrying at least one +2437C variant allele(TC+CC genotype) had a significantly increased risk for childhood Henoch-Schonlein purpura compared with the wildtype genotype (+2437TT). However,the differences between children without renal involvement in Henoch-Schonlein purpura and children with renal complications did not reach statistical significance. Conclusion:The polymorphisms of HSP70-hom+2437 T/C are genetic predisposing predictors for Henoch-Schonlein purpura in children,but has no relationship with renal complications.
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