文章摘要
姜 艺,吴 昊.长链非编码RNA MCM3AP⁃AS1在老年晚期乳腺癌患者肿瘤组织中的表达及临床病理意义[J].南京医科大学学报,2019,(11):1589~1593
长链非编码RNA MCM3AP⁃AS1在老年晚期乳腺癌患者肿瘤组织中的表达及临床病理意义
Expression and clinicopathological significance of long noncoding RNA MCM3AP⁃AS1 in tumor tissues of elderly patients with advanced breast cancer
投稿时间:2019-04-26  
DOI:10.7655/NYDXBNS201911108
中文关键词: 乳腺癌  LncRNA MCM3AP⁃AS1  Dkk1  骨转移  预后
英文关键词: breast cancer  LncRNA MCM3AP⁃AS1  Dkk1  bone metastasis  prognosis
基金项目:国家自然科学基金青年基金(81301898)
作者单位
姜 艺 南京医科大学第一临床医学院江苏 南京 210029 
吴 昊 南京医科大学第一附属医院肿瘤科江苏 南京 210029 
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中文摘要:
      目的:探讨长链非编码RNA(long noncoding RNA,LncRNA)MCM3AP?AS1在乳腺癌患者中的表达及其临床病理意义。方法:收集老年女性(年龄≥60岁)晚期乳腺癌患者肿瘤组织穿刺标本64例,应用qRT?PCR技术检测肿瘤组织中的LncRNA MCM3AP?AS1表达情况,免疫组化检测乳腺癌组织样本中的雌激素受体(estrogen receptor,ER)、孕激素受体(progesterone receptor,PR)、人表皮生长因子受体?2(human epidermal growth factor receptor?2,Her?2)、Ki?67抗原、Dickkopf相关蛋白1(Dickkopf?related protein 1,Dkk1)蛋白表达情况。Her?2免疫组化结果2+的患者进一步行荧光原位杂交试验(FISH)检测。分析LncRNA MCM3AP?AS1表达情况与乳腺癌患者临床病理之间的关系及对预后的影响。结果:组织分化差及ER、PR阴性的乳腺癌患者肿瘤组织中LncRNA MCM3AP?AS1的表达明显升高(P < 0.01)。LncRNA MCM3AP?AS1的表达与是否存在肺、肝、脑转移均无相关性,但与骨转移关系密切(P < 0.01)。激素受体阴性、Her?2 阳性、Dkk1阳性的患者更容易发生骨转移(P < 0.01)。LncRNA MCM3AP?AS1高表达的乳腺癌患者无进展生存期明显低于低表达组(P < 0.01)。结论:LncRNA MCM3AP?AS1与女性乳腺癌的恶性生物学行为关系密切。检测LncRNA MCM3AP?AS1表达对明确乳腺癌发病、转移机制及判断乳腺癌患者预后具有潜在的价值。
英文摘要:
      Objective:To investigate the expression and clinical pathological significance of long noncoding RNA(LncRNA)MCM3AP?AS1 in breast cancer patients. Methods:A total of 64 cases of tumor tissue puncture specimens were collected from elderly women(age≥60)with advanced breast cancer. The expression of LncRNA MCM3AP?AS1 in tumor tissues of these patients was detected by qRT?PCR. The expressions of ER,PR,Her?2,Ki?67 and Dkk1 protein in breast cancer tissue samples were detected by immunohistochemistry. Samples with immunohistochemical Her?2 2+ were further tested by FISH. Furthermore,we analyzed the relationship between LncRNA MCM3AP?AS1 expression and clinical pathology of breast cancer patients and its impact on prognosis. Results:The expression of LncRNA MCM3AP?AS1 was significantly increased in breast cancer patients with poor tissue differentiation and ER(-),PR(-)(P < 0.01). The expression of LncRNA MCM3AP?AS1 was not associated with the presence of liver,lung or brain metastasis,but was closely related to bone metastasis(P < 0.01). Patients with ER(-),PR(-),Her?2(+) or Dkk1(+)were more likely to have bone metastases(P < 0.01). The progression?free survival of breast cancer patients with high expression of LncRNA MCM3AP?AS1 was significantly shorter than that of low expression group(P < 0.01). Conclusion:LncRNA MCM3AP?AS1 is closely related to the malignant biological behavior of female breast cancer. The detection of LncRNA MCM3AP?AS1 expression has potential value in helping us to better understand the mechanism of breast cancer and judging the prognosis of breast cancer patients.
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