Page 39 - 南京医科大学学报自然科学版
P. 39

第42卷第10期         马牧溪,张发财,王明生,等. 基于TCGA数据库的肾透明细胞癌miRNA预后模型的构建及
                 2022年10月           蛋白互作网络分析[J]. 南京医科大学学报(自然科学版),2022,42(10):1376-1386                  ·1381 ·



                                                                      ILDR1 TUBB2B
                                                                   EYA4        DACH1
                                                                        SORCS1
                                                           NTRK2
                                                                            MAN1C1
                                                                GALNT3
                                                                                 WNT9B
                                                          BMP3
                                                    MOXD1           hsa⁃miR⁃885⁃5p  CLDN16
                                                CLDN8    OLFM3
                                                               ATP12A            ABHD17C
                                                      C7
                                            GRM1             ZNF728         KHDRBS2
                                                 SLC5A7
                                                         CADM2      KSR2  FAM81A
                                                               SEMA6D         SOWAHA
                                           LPA  BTG2
                                                            NIPAL1     SIX4
                                                                          TFAP2B
                                          CPNE4 COBLL1
                                                                      GJC1  PSMB9
                                                             CLIC5
                                           MRAP2  ALX1    FGF1   NETO1
                                                                              MARCH4
                                                     TCF21
                                              PLP1       ST6GAL1
                                                  AIF1L        TMEM92  hsa⁃miR⁃1251⁃5p
                                                     KCNJ10                     CXCL11
                                                                 TENM1        FCGR1B
                                                                     FCGR1A  NPTX2
                                         红色代表上调,绿色代表下调;三角形代表miRNA,圆形代表mRNA。
                                                  图6   miRNA与靶基因的调控网络
                                      Figure 6 The regulatory network between miRNA and target genes
                A  modulation of chemical synaptic transmission   B                postsynaptic density
                            nephron tubule development                             asymmetric synapse
                              renal tubule development                          postsynaptic specialization
                          nephron epithelium development                          glutamatergic synapse
                          kidney epithelium development                         neuron to neuron synapse
                                nephron development                                  cell⁃cell junction
                                kidney development                                 endosome membrane
                branching involved in ureteric bud morphogenesis  Count                tight junction
                            ureteric bud morphogenesis        2                 early endosome membrane      Count
                        mesonephric tubule morphogenesis      3               basolateral plasma membrane     1
                           nephron tubule morphogenesis                                                       2
                                                                                 postsynaptic membrane
                         nephron epithelium morphogenesis     4            transmembrane transporter complex
                             renal tubule morphogenesis       5                    transporter complex        3
                               nephron morphogenesis          6                                               4
                             metanephros development                  clathrin⁃coated endocytic vesicle membrane
                                kidney morphogenesis                        clathrin⁃coated endocytic vesicle  pvalue
                              ureteric bud development       pvalue          postsynaptic density membrane
                       mesonephric epithelium development                    Schaffer collateral⁃CA1 synapse  0.01
                          mesonephric tubule development      2e⁃04              Golgi cisterna membrane      0.02
                             mesonephros development                      postsynaptic specialization membrane  0.03
                                       learning               4e⁃04         clathrin⁃coated vesicle membrane
                  negative regulation of neuron apoptotic process  6e⁃04               Golgi cisterna         0.04
                  branching morphogenesis of an epithelial tube                  bicellular tight junction
                     monovalent inorganic cation homeostasis                     apical junction complex
                             potassium ion homeostasis              proteasome core complex,beta⁃subunit complex
                         metanephric nephron development                  cation⁃transporting ATPase complex
                               sodium ion homeostasis                         apicolateral plasma membrane
                            collecting duct development                          proteasome core complex
                      metanephric mesenchyme development                            connexin complex
                     magnesium ion transmembrane transport       ATPase dependent transmembrane transport complex
                                            0.04  0.06 0.08 0.10  0.12                       0.02  0.04  0.06  0.08
                                                 GeneRatio                                        GeneRatio
                C               metal ion transmembrane transporter activity  D
                                            receptor ligand activity
                                                heparin binding
                                          glycosaminoglycan binding
                                           sulfur compound binding
                DNA⁃binding transcription activator activity,RNA polymeraseⅡ⁃specific
                DNA⁃binding transcription repressor activity,RNA polymeraseⅡ⁃specific  other types of O⁃glycan biosynthesis
                                     G protein⁃coupled receptor binding  Count
                                                  lgG binding            1
                             magnesium ion sransmembrane transporter activity  2    N⁃glycan biosynthesis
                                           immunoglobulin binding
                                    palmitoyl⁃(protein)hydrolase activity  3
                               mannosyl⁃oligosaccharide mannosidase activity  4
                                    structural constituent of myelin sheath  5  pathogenic Escherichia coli infection  pvalue
                                         palmitoyl hydrolase activity                                         0.01
                               sodium:potassium⁃exchanging ATPase activity
                                   potassium⁃transporting ATPase activity  pvalue        gap junction         0.02
                                     CXCR chemokine receptor binding     0.01                                 0.03
                                            BMP receptor binding         0.02                                 0.04
                                              co⁃receptor binding                   Ras signaling pathway
                                          alpha⁃mannosidase activity     0.03
                                         gap junction channel activity   0.04
                                     lipoprotein particle receptor activity      calcium signaling pathway
                                             S100 protein binding
                                             mannosidase activity
                                            apolipoprotein binding          leukocyte transendothelial migration
                                              neuropilin binding
                           polypeptide N⁃acetylgalactosaminyltransferase activity
                     transmembrane receptor protein serine/threonine kinase binding          0    1    2    3
                                                        0.02 0.04 0.06 0.08 0.10
                                                             GeneRatio
                                A:生物过程的富集结果;B:细胞组分的富集结果;C:分子功能的富集结果;D:KEGG富集分析。
                                                      图7   靶基因的富集分析
                                              Figure 7  Enrichment analysis of target genes
   34   35   36   37   38   39   40   41   42   43   44