Page 7 - 南京医科大学学报自然科学版

第41卷第12期 南京医科大学学报（自然科学版）
2021年12月 Journal of Nanjing Medical University（Natural Sciences） ·1713 ·

·基础医学·

Alamandine 通过结合MrgD受体促进大鼠皮下脂肪间充质干细

胞成脂分化

吴晓光 ，杨传熙 ，张 晶 ，赵 锟 ，孙 伟 ，孔祥清 1*
1
2
1
1
1
南京医科大学第一附属医院心内科，江苏 南京 210029；同济大学医学院，同济大学附属杨浦医院心血管内科，上海
1 2
200090
［摘 要］ 目的：探讨血管紧张素1⁃7［angiotensin（1⁃7），Ang（1⁃7）］脱羧形成的一种肽Alamandine对大鼠脂肪来源的间充质干
细胞（adipose⁃derived mesenchymal stem cell，ADSC）成脂分化的影响，以及 Alamandine 调控成脂分化的机制。方法：用不同浓
度（0.1、1.0、10.0 μmol/L）的Alamandine处理大鼠ADSC，光镜观察脂滴大小，细胞内甘油三酯、总胆固醇测定，油红O染色法检
测成脂分化程度，Western blot检测脂肪分化相关蛋白标志物。确定最适浓度后，检测Alamandine处理大鼠ADSC不同天数的
细胞成脂分化程度。血管紧张素Ⅱ（angiotensinII，AngⅡ）和Alamandine共处理大鼠ADSC，相同方法检测成脂分化程度，探讨
Alamandine与AngⅡ对ADSC成脂分化的影响。Mas相关G蛋白偶联受体D（mas associated G protein coupled receptor D，MrgD）
拮抗剂处理大鼠ADSC，同样的方法检测成脂分化，验证Alamandine促进成脂分化的作用受体。 结果：不同浓度Alamandine处
理大鼠ADSC，发现Alamandine 促进大鼠ADSC成脂分化，且成明显剂量依赖性。选取10 μmol/L Alamandine 处理大鼠ADSC，
分别在1、3、6、10 d检测成脂分化指标，随着处理天数增加，成脂分化程度也逐渐增加且在第10天达到顶峰。AngⅡ单处理大鼠
ADSC抑制了成脂分化，而且AngⅡ减弱了Alamandine促成脂分化效应。同时MrgD拮抗剂D⁃pro7抑制了Alamandine对大鼠AD⁃
SC的促成脂分化效应。结论：Alamandine通过作用于大鼠ADSC的MrgD起到促进成脂分化的作用。
［关键词］ Alamandine；脂肪间充质干细胞；成脂分化

［中图分类号］ R329.26 ［文献标志码］ A ［文章编号］ 1007⁃4368（2021）12⁃1713⁃09
doi：10.7655/NYDXBNS20211201

Alamandine promotes adipogenic differentiation of rat subcutaneous adipose
mesenchymal stem cells by acting on MrgD receptors

1 2 1 1 1 1*
WU Xiaoguang ，YANG Chuanxi ，ZHANG Jin ，ZHAO Kun ，SUN Wei ，KONG Xiangqing
Department of Cardiology，the First Affiliated Hospital of Nanjing Medical University，Nanjing 210029；Department
1 2
of Cardiology，Tongji University School of Medicine，Yangpu Affiliated Hospital of Tongji University，Shanghai，
200090，China

［Abstract］ Objective：This study aims to investigate the effect of Alamandine，a peptide derived from the decarbonization of
angiotensin（1⁃7），on the adipogenic differentiation of adipose⁃derived mesenchymal stem cell（ADSC）in rats，and the mechanism of
Alamandine regulating lipogenic differentiation. Methods：Rat ADSCs were treated with Alamandine at different concentrations（0.1
μmol/L，1.0 μmo/L，10.0 μmol/L），lipid droplets were observed by light microscopy，intracellular triglyceride and total cholesterol
were determined，the degree of lipid differentiation was detected by oil red O staining，and the protein associated with adipogenic
differentiation was detected by Western blot. After determining the optimal concentration，10 μmol/L Alamandine was used to detect
the adipogenic differentiation degree of rat ADSCs in different days. Angiotensin Ⅱ（Ang Ⅱ）and Alamandine dealt with rats ADSCs，
same method to detect a concomitant with differentiation，detect the effect of Ang Ⅱ and Alamandine on adipogenic differentiation. Rat
ADSCs were treated with Mas associated G protein coupled receptor D（MrgD）antagonist D⁃pro7，and adipogenic differentiation was
detected by the same method，so as to further verify the role of Alamandine in promoting adipogenic differentiation. Results：

［基金项目］ 国家自然科学基金项目（81627802）；国家重点研发计划（2019YFA0210100）
∗
通信作者（Corresponding author），E⁃mail：Kongxq@njmu.edu.cn