Page 7 - 南京医科大学学报自然科学版

第42卷第4期 南京医科大学学报（自然科学版）
2022年4月 Journal of Nanjing Medical University（Natural Sciences） ·457 ·

·基础研究·

低强度脉冲超声对食源性肥胖小鼠脂肪细胞肥大的作用及机

制研究

李 禄，徐天华，舒环宇，赵云晰，孔祥清，孙 伟 *

南京医科大学第一附属医院心血管内科，江苏 南京 210029

［摘 要］ 目的：探究低强度脉冲超声（low⁃intensity pulsed ultrasound，LIPUS）对食源性肥胖小鼠脂肪细胞肥大的影响及分子
机制。方法：C57BL/6J雄性小鼠食用60% kcal脂肪的高脂饲料进行肥胖小鼠造模，肥胖小鼠接受10周腹股沟脂肪垫LIPUS辐
照。称量小鼠及靶区脂肪重量；抽取外周静脉血检测血脂、血常规和血生化；超声波穿透路径组织（皮肤、肌肉）HE染色评估
LIPUS 的体内安全性；靶区脂肪 HE 染色观察脂肪细胞大小；甘油磷酸氧化酶⁃过氧化物酶法测定靶区脂肪内甘油三酯含量；
qPCR分析靶区脂肪成脂分化转录因子、脂解酶及其转录因子的表达；Western blot检测成脂分化转录因子、脂解酶和脂解相关
信号通路的蛋白水平。结果：当声强达到77.20 mW/cm 时，LIPUS辐照明显降低肥胖小鼠靶区脂肪重量，甘油三酯含量减少，
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脂肪细胞体积减小。同时，LIPUS下调靶区脂肪成脂分化转录因子的表达，激活脂解相关信号通路，上调脂解酶的表达水平。
此外，初步结果表明 LIPUS 在该治疗剂量下具有良好的安全性。结论：LIPUS 通过降低成脂分化转录因子表达，提高脂解水
平，改善肥胖小鼠脂肪细胞肥大。
［关键词］ 低强度脉冲超声；肥胖；脂肪细胞肥大；成脂分化；脂解
［中图分类号］ R589.2 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）04⁃457⁃09
doi：10.7655/NYDXBNS20220401

Low⁃intensity pulsed ultrasound attenuates hypertrophy of local adipocytes in diet⁃induced⁃

obese mice
LI Lu，XU Tianhua，SHU Huanyu，ZHAO Yunxi，KONG Xiangqing，SUN Wei *
Department of Cardiology，the First Affiliated Hospital of Nanjing Medical University，Nanjing 210029，China

［Abstract］ Objective：The aim of this study was to investigate the potential effects and mechanisms of low ⁃ intensity pulsed
ultrasound（LIPUS）on the metabolism of hypertrophic adipose tissue induced by high⁃fat diet. Methods：C57BL/6J male mice were
fed with a high ⁃ fat diet containing 60% kcal fat for obese mice model，and then 10 obese mice were selected to receive LIPUS
irradiation at the groin fat pad for 10 weeks. The body weight of mice and inguinal fat at target area were weighed. Peripheral venous
blood was drawn for the blood lipid test，complete blood counts and plasma biochemical profile. Safety tests of LIPUS were conducted
on the basis of skin and muscle sections at LIPUS site with HE staining. And target fat stained with HE was used to observe the size of
adipocytes. The content of triglycerides in the target fat pad was measured by glycerol phosphate oxidase⁃p⁃aminophenazone methods.
The mRNA expression levels of transcription factors for adipogenic differentiation，lipolytic enzymes and their transcription factors at
the LIPUS region were analyzed by qPCR. Additionally，Western blot was applied to detect the protein levels of transcription factors for
adipogenic differentiation，lipolytic enzymes，and their related signaling pathways. Results：LIPUS irradiation significantly reduced fat
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weight，triglyceride content and adipocyte size in the target area of obese mice that received 77.20 mW/cm LIPUS treatment.
Meanwhile，LIPUS down⁃regulated the expression of transcription factors for adipogenic differentiation in the target region. Expressions
of lipolysis related signaling pathways and lipolytic enzymes were up⁃regulated via LIPUS stimulation. In addition，preliminary results
indicate that LIPUS has a good safety profile at current dose. Conclusion：LIPUS diminishes local adipose tissue hypertrophy induced
by high⁃fat diet through inhibiting adipogenic differentiation and encouraging lipolysis procedure.

［基金项目］ 国家重大科研仪器研制项目（81627802）
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通信作者（Corresponding author），E⁃mail：weisun7919@njmu.edu.cn