Page 36 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第42卷第9期
·1230 · Journal of Nanjing Medical University（Natural Sciences） 2022年9月

·基础研究·

东莨菪碱对小鼠边界细胞空间信息编码的影响

何 雷
东南大学生命科学与技术学院，江苏 南京 210096

［摘 要］ 目的：探讨东莨菪碱对小鼠边界细胞空间信息编码及空间记忆能力的影响。方法：将健康雄性2月龄C57BL/6小
鼠随机分为生理盐水对照组和东莨菪碱组，使用 morris 水迷宫实验分析两组的空间记忆能力，随后向压后皮质（retrosplenial
cortex，RSC）植入32通道微丝电极；手术后恢复7 d，进行旷场实验并同时记录电信号，通过计算分离出边界细胞。随后通过腹
腔给药方式，给予小鼠25 μg/kg 东莨菪碱或等体积的生理盐水，24 h后进行旷场实验并同时记录电信号，与给药前记录到的边
界细胞的放电信号进行对比。结果：生理盐水对照组和东莨菪碱组水迷宫实验的学习曲线差异有统计学意义（P < 0.05），目标
象限时间、穿越平台次数差异均有统计学意义（P < 0.05）。注射东莨菪碱或生理盐水24 h后，小鼠旷场总路程差异无统计学意
义（P > 0.05）；注射东莨菪碱对小鼠边界细胞放电频率无显著性影响（P > 0.05）；注射东莨菪碱后，边界细胞陆地移动距离
（earth mover’s distance，EMD）显著增加（P < 0.05），而注射生理盐水无明显影响（P > 0.05）。结论：东莨菪碱可能是通过影响小
鼠边界细胞编码从而影响其空间记忆能力的。
［关键词］ 东莨菪碱；压后皮质；边界细胞；旷场
［中图分类号］ R338 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）09⁃1230⁃05
doi：10.7655/NYDXBNS20220905

Effects of scopoplamine on boundary coding of border cells in mice

HE Lei
School of Life Sciences and Technology，Southeast University，Nanjing 210096，China

［Abstract］ Objective：This study aims to investigate how scopolamine affects on boundary coding of border cells and spatial memory
of mice. Methods：Healthy male 2⁃month⁃old C57BL/6 mice were randomly divided into saline control group and scopolamine group.
Morris water maze was used to evaluate spatial memory ability. Then 32 channel microwire electrodes were implanted into retrosplenial
cortex（RSC）. After 7 days of recovery，the open field experiment was carried out and the electrical signals were recorded at the same
time. Border cells were isolated after analysis. Then 25 μg/kg scopolamine or equivalent volume of normal saline was intraperitoneally
injected. After 24 hours，the open field experiment was carried out and the electrical signals were recorded at the same time. The
changes of border cells recorded before and after treatment were analyzed. Results：There were significant differences in the learning
curve of water maze experiment between saline control group and scopolamine group（P < 0.05），the time in the target quadrant（P <
0.05）and the number of crossing platform（P < 0.05）. After intraperitoneal injection of scopolamine or normal saline for 24 hours，the
total distance of mice in open field had no significant difference（P > 0.05）. Injection of scopolamine had no significant effect on the
firing frequency of border cells in mice（P > 0.05）. The earth mover’s distance（EMD）was significantly increased after scopolamine
injection（P < 0.05），while normal saline injection had no significant effect on EMD（P > 0.05）. Conclusion：Scopolamine may affect
the ability of spatial memory by affecting boundary coding of border cells in mice.
［Key words］ scopolamine；retrosplenial cortex；border cell；open field test
［J Nanjing Med Univ，2022，42（09）：1230⁃1234］

多项临床研究表明，小剂量东莨菪碱治疗抑郁 力。动物实验表明，应用东莨菪碱会降低空间定位
症时有快速抗抑郁的效应 ［1-5］ ，但会对认知及学习记 的学习能力，表现异常的空间记忆唤起，对新环境
忆功能产生一定的不良反应，特别是空间定位能 的探究时间延长和认知能力下降等，但其作用机制