Page 23 - 南京医科大学学报自然科学版

第41卷第11期 南京医科大学学报（自然科学版）
2021年11月 Journal of Nanjing Medical University（Natural Sciences） ·1585 ·

·基础研究·

润滑障碍中的lncRNA⁃mRNA共表达网络的构建及功能预测

刘天娇 ，张晶晶 ，马洁桦 ，潘连军 ，阮红杰 5*
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1 南京医科大学附属妇产医院妇女保健科，产科，医学研究中心，泌尿外科，急诊医学科，江苏 南京 210004
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［摘 要］ 目的：通过构建阴道润滑障碍（lubrication disorder，LD）女性阴道上皮组织差异表达lncRNA⁃mRNA共表达网络，探
索 LD 患者的潜在发病机制。方法：利用以|Log2FC|≥2 且校正后 P 值＜0.05，鉴别 LD 和正常对照组之间的长链非编码 RNA
（long non⁃coding RNA，LncRNA）以及mRNA的表达谱，利用Cytoscape软件构建差异表达lncRNA及差异表达mRNA网络，采用
Gene Ontology（GO）和KEGG Pathway分析共表达网络中mRNA的生物学功能。结果：通过下一代测序技术，根据|Log2FC|≥2且
校正后P值＜0.05标准筛选出LD组和正常对照组中共计499条上调表达的lncRNA、337条下调表达的lncRNA，以及1 582条
上调表达mRNA、633条下调表达的mRNA。随后，通过其中100个lncRNA 与311个mRNA构建了基于差异表达的lncRNA 和
mRNA的共表达网络。最后，共表达网络的功能富集分析表明mRNA主要与心肌相关的疾病和功能有关。结论：LD的发病机
制可能与血液循环功能障碍、局部肌肉功能障碍以及cGMP通路等有关，需要相关研究来进一步证实。
［关键词］ 阴道润滑障碍；长链非编码RNA；共表达网络
［中图分类号］ R711.73 ［文献标志码］ A ［文章编号］ 1007⁃4368（2021）1⁃1585⁃07
doi：10.7655/NYDXBNS20211104

Construction and function prediction of lncRNA ⁃ mRNA co ⁃ expression network in
lubrication disorder

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LIU Tianjiao ，ZHANG Jingjing ，MA Jiehua ，PAN Lianjun ，RUAN Hongjie 5*
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1 Department of Women’s Health Care，Obstetrics Department，Medical Research Center，Department of Urology，
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5 Department of Emergency，the Affiliated Obstetrics and Gynaecology Hospital of Nanjing Medical University，Nanjing
210004，China
［Abstract］ Objective： This study aims to use bioinformatics methods to screen the differentially long non⁃coding RNA（LncRNA）in
vaginal epithelial tissues of women with vaginal lubrication disorder（LD），and to construct patients with vaginal lubrication disorder
based on the hypothesis of lncRNA⁃mRNA co⁃expression network to further explore the potential pathogenesis of patients with vaginal
LD，in order to provide new ideas for the diagnosis and treatment of patients with vaginal LD. Methods：Using |Log2FC|≥2 and correct
P ralue＜0.05，the expression profile of long⁃chain non⁃coding RNA and mRNA between LD group and normal control group were
identified. Using Cytoscape software，a lncRNA⁃mRNA network of candidate lncRNAs was constructed. Using Gene Ontology（GO）and
KEGG Pathway，the biological functions of mRNAs in co⁃expression network were analyzed. Results：A total of 499 up⁃regulated
lncRNAs，337 down⁃regulated lncRNAs，and 1 582 up⁃regulated mRNAs，633 were selected in the LD group and the normal control
group based on the next ⁃ generation sequencing technology according to the criteria of |Log 2FC| ≥2 and correct P ralue＜0.05.
Subsequently，a lncRNA⁃mRNA co⁃expression network was constructed based on differentially expressed lncRNA and mRNA through
100 lncRNAs and 311 mRNAs. Finally，the functional enrichment analysis of the lncRNA⁃mRNA network showed that mRNA was
mainly related to myocardial⁃related diseases and functions. Conclusion：The pathogenesis of LD may be related to blood circulation
dysfunction，local muscle dysfunction，and cGMP pathway，etc. This requires further studies at the cellular level，animal level and even
human level to confirm.
［Key words］ vaginal lubrication disorder；long non⁃coding RNA；co⁃expression network
［J Nanjing Med Univ，2021，41（11）：1585⁃1591］

［基金项目］ 国家自然科学基金项目（81771572）
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通信作者（Corresponding author），E⁃mail： hongjie_ruan@126.com