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第41卷第12期 许 耿,顾海涛. 心脏类器官的研究进展及其临床应用[J].
2021年12月 南京医科大学学报(自然科学版),2021,41(12):1837-1842 ·1841 ·
在生长因子或分子抑制剂,可能会影响到其药物实 2015,15(2):365-375
验的结果;第三,目前的心脏类器官构建方案众多, [11] HOFBAUER P,JAHNEL S M,PAPAI N,et al. Cardioids
标准不一,高效、高质量、标准化的心脏类器官的生 reveal self⁃organizing principles of human cardiogenesis
[J]. Cell,2021,184(12):3299-3317
成较为困难;第四,现有的心脏类器官仍不成熟,
[12] RICHARDS D J,LI Y,KERR C M,et al. Human cardiac
形成的心肌细胞处于胎儿或新生儿的状态 [47] 。
organoids for the modelling of myocardial infarction and
尽管目前这项技术仍存在一些缺陷,但其在疾病 drug cardiotoxicity[J]. Nat Biomed Eng,2020,4(4):
机 制 研 究 及 相 应 药 物 试 验 等 方 面 有 着 巨 大 潜 446-462
力。随着类器官技术的进一步完善与发展,将加 [13] ZIMMERMANN W H,DIDIÉ M,DÖKER S,et al. Heart
快心脏药物的研发和实现心脏疾病的个性化治 muscle engineering:an update on cardiac muscle replace⁃
疗,类器官技术也可能成为器官移植的一个新的 ment therapy[J]. Cardiovasc Res,2006,71(3):419-429
发展方向。 [14] ASNES C F,MARQUEZ J P,ELSON E L,et al. Reconsti⁃
tution of the Frank ⁃ Starling mechanism in engineered
[参考文献]
heart tissues[J]. Biophys J,2006,91(5):1800-1810
[1] ROTH G A,JOHNSON C,ABAJOBIR A,et al. Global,re⁃ [15] RADISIC M,PARK H,SHING H,et al. Functional assem⁃
bly of engineered myocardium by electrical stimulation of
gional,and national burden of cardiovascular diseases for
cardiac myocytes cultured on scaffolds[J]. Proc Natl
10 causes,1990 to 2015[J]. J Am Coll Cardiol,2017,70
Acad Sci U S A,2004,101(52):18129-18134
(1):1-25
[16] HE B,LI G,ZHANG X. Noninvasive three⁃dimensional
[2] RUAN Y,GUO Y,ZHENG Y,et al. Cardiovascular dis⁃
activation time imaging of ventricular excitation by means
ease(CVD)and associated risk factors among older adults
of a heart⁃excitation model[J]. Phys Med Biol,2002,47
in six low ⁃ and middle ⁃ income countries:results from
SAGE Wave 1[J]. BMC Public Health,2018,18(1): (22):4063-4078
[17] SHADRIN I Y,ALLEN B W,QIAN Y,et al. Cardiopatch
778-789
platform enables maturation and scale⁃up of human plu⁃
[3] ZHOU J,SU J,FU X,et al. Microfluidic device for prima⁃
ripotent stem cell ⁃ derived engineered heart tissues[J].
ry tumor spheroid isolation[J]. Exp Hematol Oncol,
Nat Commun,2017,8(1):18-25
2017,6(22):64-81
[18] SHKUMATOV A,BAEK K,KONG H. Matrix rigidity ⁃
[4] BEN⁃DAVID U,HA G,TSENG Y Y,et al. Patient⁃de⁃
modulated cardiovascular organoid formation from embry⁃
rived xenografts undergo mouse⁃specific tumor evolution
oid bodies[J]. PLoS One,2014,9(4):47-64
[J]. Nat Genet,2017,49(11):67-75
[19] LEE E J,KIM D E,AZELOGLU E U,et al. Engineered
[5] ROSSI G,MANFRIN A,LUTOLF M P. Progress and po⁃
cardiac organoid chambers:toward a functional biological
tential in organoid research[J]. Nat Rev Genet,2018,19
model ventricle[J]. Tissue Eng Part A,2008,14(2):
(11):671-687
215-225
[6] LANCASTER M A,KNOBLICH J A. Organogenesis in a
[20] MA Z,WANG J,LOSKILL P,et al. Self⁃organizing human
dish:modeling development and disease using organoid
cardiac microchambers mediated by geometric confine⁃
technologies[J]. Science,2014,345(6194):124-137
ment[J]. Nat Commun,2015,6(7):413-423
[7] XU H,JIAO Y,QIN S,et al. Organoid technology in dis⁃
[21] ANDERSEN P,TAMPAKAKIS E,JIMENEZ D V,et al.
ease modelling,drug development,personalized treatment
Precardiac organoids form two heart fields via Bmp/Wnt
and regeneration medicine[J]. Exp Hematol Oncol,2018, signaling[J]. Nat Commun,2018,9(1):31-40
7(30):133-145 [22] HOANG P,WANG J,CONKLIN B R,et al. Generation of
[8] 许 磊,唐晓艳,徐 敏,等. 人类 3D 大脑类器官研究 spatial⁃patterned early⁃developing cardiac organoids us⁃
进展[J]. 南京医科大学学报(自然科学版),2020,40 ing human pluripotent stem cells[J]. Nat Protoc,2018,13
(1):133-140 (4):723-737
[9] ASHOK A,CHOUDHURY D,FANG Y,et al. Towards [23] XIN M,OLSON E N,BASSEL⁃DUBY R. Mending broken
manufacturing of human organoids[J]. Biotechnol Adv, hearts:cardiac development as a basis for adult heart re⁃
2020,39(10):23-35 generation and repair[J]. Nat Rev Mol Cell Biol,2013,14
[10] CHEN V C,YE J,SHUKLA P,et al. Development of a (8):529-541
scalable suspension culture for cardiac differentiation [24] WALDO K L,HUTSON M R,WARD C C,et al. Second⁃
from human pluripotent stem cells[J]. Stem Cell Res, ary heart field contributes myocardium and smooth mus⁃