Page 29 - 南京医科大学学报自然科学版
P. 29
第42卷第10期 南京医科大学学报(自然科学版)
2022年10月 Journal of Nanjing Medical University(Natural Sciences) ·1371 ·
·基础医学·
葛根素抑制支气管哮喘小鼠气道炎症和TSLP 介导的Th2免疫
作用的研究
王文璐 ,何 权 ,周林福 2*
2
1,2
南京大学医学院附属泰康仙林鼓楼医院发热门诊,江苏 南京 210093;南京医科大学第一附属医院呼吸与危重症医学科,
1 2
江苏 南京 210029
[摘 要] 目的:研究葛根素对慢性哮喘小鼠模型气道炎症和Th2免疫的影响及其机制。方法:将BALB/c小鼠32只随机均分
为正常组、哮喘组、葛根素组和布地奈德组,每组8只。除正常组外,其余各组采用卵白蛋白(ovabumin,OVA)致敏和激发,连
续12周,建立慢性哮喘小鼠模型。末次激发24 h后,使用小鼠肺功能仪进行气道反应性测定,苏木精⁃伊红(HE)染色观察肺组
织炎性细胞浸润情况,过碘酸雪夫(PAS)染色观察杯状细胞增生情况,用酶联免疫吸附测定(ELISA)法检测 4 组小鼠血清总
IgE和支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中Th2细胞因子(白介素⁃4、白介素⁃13)水平。Western blot观察肺
组织胸腺基质淋巴细胞生成素(thymic stromal lymphopoietin,TSLP)的蛋白表达。结果:与正常组比较,葛根素组、布地奈德组、
哮喘组气道阻力、气道炎症,血清总IgE,BALF中白介素⁃4、白介素⁃13以及肺组织TSLP蛋白表达明显增高(P < 0.05)。与哮喘
组比较,葛根素组、布地奈德组各项观测指标均明显降低(P < 0.05)。葛根素组和布地奈德组气道PAS阳性上皮细胞数/支气
管较哮喘组下降(P < 0.05)。结论:葛根素抑制慢性哮喘小鼠气道炎症、气道高反应性和Th2免疫,这可能与抑制TSLP的表达
有关。
[关键词] 葛根素;哮喘;气道炎症;Th2免疫;胸腺基质淋巴细胞生成素
[中图分类号] R562.2 [文献标志码] A [文章编号] 1007⁃4368(2022)10⁃1371⁃06
doi:10.7655/NYDXBNS20221004
The inhibitory effects of puerarin on airway inflammation and TSLP⁃induced Th2 immunity
in a murine model of chronic asthma
1,2
2
WANG Wenlu ,HE Quan ,ZHOU Linfu 2*
Fever Clinic,Affiliated Taikang Xianlin Drum Tower Hospital,Medical School of Nanjing University,Nanjing
1
2
210093;Department of Respiratory and Critical Care Medicine,the First Affiliated Hospital of Nanjing Medical
University,Nanjing 210029,China
[Abstract] Objective:To investigate the inhibitory effects of puerarin on airway inflammation and Th2 immunity in a murine model
of chronic asthma. Methods:Thirty⁃two female BALB/c mice were randomly divided into four groups,including the control group,the
ovabumin(OVA)group,the puerarin group,and the budesonide group,8 mice in each group. In the latter three groups,chronic
asthmatic models were established by OVA allergy and activation for 12 weeks. Twenty⁃four hours after the last airway provocation,
acetylcholine(Ach)was administered via caudalis vein to measure airway resistance by pulmonary function detector. The airway
inflammation and hypersecretion in the lung were assessed by hematoxylin & eosin(HE)and periodic acid Schiff(PAS)staining,
respectively. The levels of interleukin(IL)⁃4 and IL⁃13 in bronchoalveolar lavage fluid(BALF)and total immunoglobulin E(IgE)in
serum were measured by ELISA. The protein expression of thymic stromal lymphopoietin(TSLP)was determined by Western blot.
Results:As compared with the control group,the airway hyperreactivity,airway inflammation,cell count and eosinophil percentage in
[基金项目] 国家重点研发计划(2018YFC1313600);国家自然科学基金重点国际(地区)合作研究(81820108001);国家自然
科学基金(81670029);江苏省医学重点人才项目(ZDRCA2016018);江苏省“333”高层次人才工程第二层次培养对象
(BRA2019078)
∗
通信作者(Corresponding author),E⁃mail:lfzhou@njmu.edu.cn