Page 69 - 南京医科大学学报自然科学版

第42卷第7期 南京医科大学学报（自然科学版）
2022年7月 Journal of Nanjing Medical University（Natural Sciences） ·965 ·

·基础研究·

生物降解Mg增强PLA复合膜用于引导骨再生的抗菌性能研究

王 萍 ，王先丽 ，吴 迪 ，白 晶 ，路萌萌 ，汤春波 1*
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南京医科大学附属口腔医院口腔种植科，江苏省口腔疾病研究重点实验室，江苏省口腔转化医学工程研究中心，江苏 南
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京 210029；江苏省东南大学先进金属材料重点实验室，江苏 南京 211189
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［摘 要］ 目的：探讨生物可降解镁（magnesium，Mg）增强聚乳酸（polylactic acid，PLA）复合膜的抗菌功能。方法：将不同比例
的镁颗粒负载于聚乳酸基质中制备Mg/PLA复合膜，用于引导骨再生（guided bone regeneration，GBR）。利用扫描电子显微镜观
察样品表面形貌，电子万能试验机检测复合材料力学性能变化，并测试材料24 h内于磷酸盐缓冲液中降解引起的pH变化。同
时，用MC3T3⁃E1细胞系、L929细胞系以CCK⁃8法检测材料降解产物的生物相容性。用扫描电子显微镜、细菌平板计数观察大
肠杆菌和金黄色葡萄球菌在材料表面的生长情况。结果：10%wt和 20%wt的镁添加量增强了材料的力学强度，而Mg含量过高
（40%wt）则削弱了其对 PLA 机械性能的增强作用。无论是 PLA 还是任一复合材料的降解产物都表现出良好的生物相容性。
而且，20%wt Mg/PLA和40%wt Mg/PLA复合膜具有明显的抗菌作用，且抑菌率随着Mg含量的增加而提高。结论：一定Mg负载
量的Mg/PLA复合材料对聚合物机械性能和抗菌作用有较好的改善，有望作为屏障膜材料应用于口腔组织缺损的治疗。
［关键词］ 复合膜；聚乳酸；镁；抗菌；骨再生
［中图分类号］ R783.1 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）07⁃965⁃08
doi：10.7655/NYDXBNS20220708

Research on antibacterial properties of the biodegradable magnesium reinforced polylactic
acid composite membrane for guided bone regeneration

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WANG Ping ，WANG Xianli ，WU Di ，BAI Jing ，LU Mengmeng ，TANG Chunbo 1*
1 Department of Dental Implant and Prosthodontics，the Affiliated Stomatological Hospital of Nanjing Medical
University，Jiangsu Province Key Laboratory of Oral Diseases，Jiangsu Province Engineering Research Center of
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Stomatological Translational Medicine，Nanjing 210029；Jiangsu Key Laboratory for Advanced Metallic Materials，
Southeast University，Nanjing 211189，China
［Abstract］ Objective：This study aims to investigate the antibacterial function of biodegradable magnesium（Mg） reinforced
polylactic acid（PLA）composite membranes. Methods：In the present study，Mg/PLA composite membranes were prepared by loading
different proportions of Mg particles into PLA matrix for guided bone regeneration（GBR）. The surface morphology of samples were
observed by scanning electron microscope，and the mechanical performance of the composites was detected by the electro⁃mechanical
universal testing machines. We immersed the material in phosphate buffered saline（PBS）and detected the pH variation during the
degradation of 24 h. MC3T3⁃E1 cell line，L929 cell line were used to detect biocompatibility of the material degradation product by
CCK⁃8 method. Besides，we observe the growth of E.coli and S.aureus on the surface of material with scanning electron microscopy and
bacterial plate counting. Results：Results showed that 10%wt and 20% wt of Mg ehanced the mechanical strength of the material，
however，the high Mg content（40%wt）weekened the enhancement effect of Mg particles on the mechanical properties of PLA. The
degradation products of PLA or any of the composites showed good biocompatibility. And 20% wt Mg/PLA and 40% wt Mg/PLA
composite membranes had obvious antibacterial functions，and the antibacterial rate increased with the increase of Mg content.
Conclusion：Mg/PLA composite material has a good improvement on the mechanical properties and antibacterial effect of polymer，

［基金项目］ 国家自然科学基金（82170993）；东南大学 -南京医科大学合作研究项目（2242018K3DN03）；政府间国际科技创
新合作重点专项（2018YFE0194100）
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通信作者（Corresponding author），E⁃mail：cbtang@njmu.edu.cn