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·202 · 南 京 医 科 大 学 学 报 2023年2月
A
siFAT1+miR⁃ siNC+miR⁃
siNC+IC#1 siFAT1+IC#1 181d⁃5p inhibitor 181d⁃5p inhibitor
1.72% 3.02% 4.77% 22.8% 1.79% 12.79% 1.33% 1.66%
10 4 10 4 10 4 10 4
HSC⁃3 PI PI PI PI
10 3 10 3 10 3 10 3
90.26% 5% 69.79% 2.64% 82.29% 3.13% 94.54% 2.46%
10 2 10 3 10 4 10 2 10 3 10 4 10 2 10 3 10 4 10 2 10 3 10 4
FITC⁃H FITC⁃H FITC⁃H FITC⁃H
FAT1+miR⁃ NC+miR⁃
NC+MC#1 FAT1+MC#1 181d⁃5p mimic 181d⁃5p mimic
1.76% 6.04% 2.22% 0.94% 2.35% 11.48% 1.82% 18.5%
10 4 10 4 10 4 10 4
SCC⁃25 PI PI PI PI
10 3 10 3 10 3 10 3
88.96% 3.24% 93.39% 3.44% 82.50% 3.67% 75.86% 3.82%
10 2 10 3 10 4 10 2 10 3 10 4 10 2 10 3 10 4 10 2 10 3 10 4
FITC⁃H FITC⁃H FITC⁃H FITC⁃H
siFAT1+miR⁃ siNC+miR⁃
siNC+IC#2 siFAT1+IC#2 199a⁃5p inhibitor 199a⁃5p inhibitor
0.97% 5.6% 4.63% 19.43% 3.48% 12.58% 5.34% 1.35%
10 4 10 4 10 4 10 4
HSC⁃3 PI PI PI PI
10 3 10 3 10 3 10 3
91.25% 2.18% 71.32% 4.63% 82.28% 1.66% 90.72% 2.59%
10 2 10 3 10 4 10 2 10 3 10 4 10 2 10 3 10 4 10 2 10 3 10 4
FITC⁃H FITC⁃H FITC⁃H FITC⁃H
FAT1+miR⁃ NC+miR⁃
NC+MC#2 FAT1+MC#2 199a⁃5p mimic 199a⁃5p mimic
1.4% 3.38% 0.51% 0.56% 2.38% 14.77% 1.73% 12.33%
10 4 10 4 10 4 10 4
SCC⁃25 PI PI PI PI
10 3 10 3 10 3 10 3
89.45% 2.18% 95.28% 3.65% 80.51% 2.34% 72.06% 4.19%
10 2 10 3 10 4 10 2 10 3 10 4 10 2 10 3 10 4 10 2 10 3 10 4
FITC⁃H FITC⁃H FITC⁃H FITC⁃H
（ % ） （ % ）
B （ % ） （ % ）
HSC⁃3中细胞凋亡率 30 0 SCC⁃25中细胞凋亡率 30 0 HSC⁃3中细胞凋亡率 30 0 SCC⁃25中细胞凋亡率 30 0
20
20
20
20
10
10
10
10
siFAT1+miR⁃181d⁃5p inhibitor FAT1+miR⁃181d⁃5p mimic siFAT1+miR⁃199a⁃5p inhibitor FAT1+miR⁃199a⁃5p mimic
siNC+IC#1
NC+miR⁃199a⁃5p mimic
siFAT1+IC#1
NC+MC#2
FAT1+MC#2
NC+MC#1
NC+miR⁃181d⁃5p imimic
FAT1+MC#1
siNC+IC#2
siFAT1+IC#2
siNC+miR⁃181d⁃5p inhibitor
siNC+miR⁃199a⁃5p inhibitor
A：流式细胞术；B：细胞凋亡结果。
图4 circFAT1通过调控miR⁃181d⁃5p/miR⁃199a⁃5p调节OSCC细胞凋亡
Figure 4 circFAT1 regulates OSCC cell apoptosis by regulating miR⁃181d⁃5p/miR⁃199a⁃5p
