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第42卷第10期 马牧溪，张发财，王明生，等. 基于TCGA数据库的肾透明细胞癌miRNA预后模型的构建及
2022年10月 蛋白互作网络分析［J］. 南京医科大学学报（自然科学版），2022，42（10）：1376-1386 ·1381 ·

ILDR1 TUBB2B
EYA4 DACH1
SORCS1
NTRK2
MAN1C1
GALNT3
WNT9B
BMP3
MOXD1 hsa⁃miR⁃885⁃5p CLDN16
CLDN8 OLFM3
ATP12A ABHD17C
C7
GRM1 ZNF728 KHDRBS2
SLC5A7
CADM2 KSR2 FAM81A
SEMA6D SOWAHA
LPA BTG2
NIPAL1 SIX4
TFAP2B
CPNE4 COBLL1
GJC1 PSMB9
CLIC5
MRAP2 ALX1 FGF1 NETO1
MARCH4
TCF21
PLP1 ST6GAL1
AIF1L TMEM92 hsa⁃miR⁃1251⁃5p
KCNJ10 CXCL11
TENM1 FCGR1B
FCGR1A NPTX2
红色代表上调，绿色代表下调；三角形代表miRNA，圆形代表mRNA。
图6 miRNA与靶基因的调控网络
Figure 6 The regulatory network between miRNA and target genes
A modulation of chemical synaptic transmission B postsynaptic density
nephron tubule development asymmetric synapse
renal tubule development postsynaptic specialization
nephron epithelium development glutamatergic synapse
kidney epithelium development neuron to neuron synapse
nephron development cell⁃cell junction
kidney development endosome membrane
branching involved in ureteric bud morphogenesis Count tight junction
ureteric bud morphogenesis 2 early endosome membrane Count
mesonephric tubule morphogenesis 3 basolateral plasma membrane 1
nephron tubule morphogenesis 2
postsynaptic membrane
nephron epithelium morphogenesis 4 transmembrane transporter complex
renal tubule morphogenesis 5 transporter complex 3
nephron morphogenesis 6 4
metanephros development clathrin⁃coated endocytic vesicle membrane
kidney morphogenesis clathrin⁃coated endocytic vesicle pvalue
ureteric bud development pvalue postsynaptic density membrane
mesonephric epithelium development Schaffer collateral⁃CA1 synapse 0.01
mesonephric tubule development 2e⁃04 Golgi cisterna membrane 0.02
mesonephros development postsynaptic specialization membrane 0.03
learning 4e⁃04 clathrin⁃coated vesicle membrane
negative regulation of neuron apoptotic process 6e⁃04 Golgi cisterna 0.04
branching morphogenesis of an epithelial tube bicellular tight junction
monovalent inorganic cation homeostasis apical junction complex
potassium ion homeostasis proteasome core complex，beta⁃subunit complex
metanephric nephron development cation⁃transporting ATPase complex
sodium ion homeostasis apicolateral plasma membrane
collecting duct development proteasome core complex
metanephric mesenchyme development connexin complex
magnesium ion transmembrane transport ATPase dependent transmembrane transport complex
0.04 0.06 0.08 0.10 0.12 0.02 0.04 0.06 0.08
GeneRatio GeneRatio
C metal ion transmembrane transporter activity D
receptor ligand activity
heparin binding
glycosaminoglycan binding
sulfur compound binding
DNA⁃binding transcription activator activity，RNA polymeraseⅡ⁃specific
DNA⁃binding transcription repressor activity，RNA polymeraseⅡ⁃specific other types of O⁃glycan biosynthesis
G protein⁃coupled receptor binding Count
lgG binding 1
magnesium ion sransmembrane transporter activity 2 N⁃glycan biosynthesis
immunoglobulin binding
palmitoyl⁃（protein）hydrolase activity 3
mannosyl⁃oligosaccharide mannosidase activity 4
structural constituent of myelin sheath 5 pathogenic Escherichia coli infection pvalue
palmitoyl hydrolase activity 0.01
sodium：potassium⁃exchanging ATPase activity
potassium⁃transporting ATPase activity pvalue gap junction 0.02
CXCR chemokine receptor binding 0.01 0.03
BMP receptor binding 0.02 0.04
co⁃receptor binding Ras signaling pathway
alpha⁃mannosidase activity 0.03
gap junction channel activity 0.04
lipoprotein particle receptor activity calcium signaling pathway
S100 protein binding
mannosidase activity
apolipoprotein binding leukocyte transendothelial migration
neuropilin binding
polypeptide N⁃acetylgalactosaminyltransferase activity
transmembrane receptor protein serine/threonine kinase binding 0 1 2 3
0.02 0.04 0.06 0.08 0.10
GeneRatio
A：生物过程的富集结果；B：细胞组分的富集结果；C：分子功能的富集结果；D：KEGG富集分析。
图7 靶基因的富集分析
Figure 7 Enrichment analysis of target genes