Page 45 - 南京医科大学学报自然科学版

第42卷第12期 南京医科大学学报（自然科学版）
2022年12月 Journal of Nanjing Medical University（Natural Sciences） ·1681 ·

·肿瘤专题研究·

DAAM基因家族在胰腺癌中的表达及其与免疫检查点的相关性

梅 杰，许隽颖 ，顾丁一，王惠宇，刘超英
*
南京医科大学附属无锡人民医院肿瘤科，江苏 无锡 214023

［摘 要］ 目的：探究蓬乱蛋白相关形态形成活化因子（dishevelled⁃associated activator of morphogenesis，DAAM）1和DAAM2在
胰腺癌中的表达及临床意义。方法：购买胰腺癌组织芯片（HPanA120Su02），包含66例癌及54例癌旁样本；采用免疫组织化学法
检测 DAAM1、DAAM2 在胰腺癌及癌旁组织中的表达水平；下载癌症基因组图谱（the Cancer Genome Atlas，TCGA）数据库中
DAAM1、DAAM2及细胞程序性死亡配体1（programmed cell death ligand 1，PDL1）等免疫检查点分子的表达谱数据；采用t检验、
χ 检验、log⁃rank检验、相关性分析等统计学方法分别分析DAAM1、DAAM2在癌与癌旁组织间表达的差异，二者的共表达关系，与
2
临床病理参数、预后的关系，及其与免疫检查点分子表达的相关性。结果：相较于癌旁组织，DAAM1、DAAM2在胰腺癌中的表达
均显著上调（P < 0.001），且胰腺癌组织中DAAM1与DAAM2的表达呈显著正相关（P < 0.001）；DAAM1表达水平与肿瘤分化程度
（P=0.062）和患者生存状态（P=0.061）存在一定相关性，但差异无统计学意义，与其他临床病理参数无显著相关性（P > 0.05），
而DAAM2表达水平与各项临床病理参数均无显著相关性（P > 0.05）；TCGA数据库中，DAAM1及DAAM2与PDL1等多个免疫
检查点的表达呈显著正相关（P < 0.001）。结论：DAAM1、DAAM2在胰腺癌中的表达显著上调，与PDL1等免疫检查点的表达
呈正相关，可能是胰腺癌发生、发展及免疫逃避的关键调控因素。
［关键词］ DAAM1；DAAM2；胰腺癌；预后；免疫检查点
［中图分类号］ R735.9 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）12⁃1681⁃06
doi：10.7655/NYDXBNS20221206

Expression of DAAM gene family in pancreatic cancer and its correlations with immune
checkpoints

*
MEI Jie，XU Junying ，GU Dingyi，WANG Huiyu，LIU Chaoying
Department of Oncology，Wuxi People’s Hospital Affiliated to Nanjing Medical University，Wuxi 214023，China

［Abstract］ Objective：To explore the expression and clinical significance of dishevelled⁃associated activator of morphogenesis 1
（DAAM1）and DAAM2 in pancreatic cancer. Methods：Pancreatic cancer tissue microarray（TMA，HPanA120Su02）was obtained from
OUTDO BioTech（Shanghai），including 66 tumor samples and 54 adjacent samples. Immunohistochemistry（IHC）was used to detect
the expression levels of DAAM1 and DAAM2 in pancreatic cancer and para⁃tumor tissues. Expression profiles of DAAM1，DAAM2，
2
PDL1 and other immune checkpoints were downloaded from the TCGA database. Statistical methods，such as t⁃test，χ test，log⁃rank
test and correlation analysis，were used to analyze the differential expression of DAAM1 and DAAM2 between tumor and adjacent
samples，the co⁃expression pattern of DAAM1 and DAAM2，and their association with clinicopathological parameters，prognosis and
expression levels of immune checkpoints. Results：Compared with the adjacent tissues，the expression of DAAM1 and DAAM2 in
pancreatic cancer were significantly upregulated（P < 0.001），and the expression levels of DAAM1 and DAAM2 in pancreatic cancer
tissue were significantly positively correlated（P < 0.001）. There was marginally significant in the correlation between DAAM1
expression and tumor differentiation（P=0.062）and survival status（P=0.061），but DAAM1 expression was not significantly correlated
with other clinic⁃pathological parameters（P > 0.05）. However，DAAM2 expression was not related to any clinic⁃pathological parameters
（P > 0.05）. In the TCGA database，DAAM1 and DAAM2 were positively correlated with the expression levels of multiple immune
checkpoints（P < 0.001）. Conclusion：The expression of DAAM1 and DAAM2 are significantly upregulated in pancreatic cancer，and

［基金项目］ 江苏省六大人才高峰项目（WSN186）；无锡市科技发展资金项目（N20202018）；太湖人才计划（2020005）；江苏省
研究生科研创新计划项目（KYCX21_1559）；江苏省博士后科研资助计划
∗
通信作者（Corresponding author），E⁃mail：doctorxyj123@163.com