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·1742 · 南 京 医 科 大 学 学 报 2025年12月

2.3 uc004coz.1影响细胞增殖迁移 的迁移能力较对照组明显减弱（P < 0.01，图 3C、
si⁃uc004coz.1 明显减弱 HUVEC 内 uc004coz.1 D）。
的表达（P=0.001，图 3A）。CCK⁃8 实验显示敲减 2.4 uc004coz.1影响细胞增殖水平
HUVEC 内 uc004coz.1 后，24、48、72 h HUVEC 增殖 EdU 实验结果显示 uc004coz.1 敲减后细胞 EdU
能力较对照组下降（P=0.01，P < 0.01，P=0.02，图 阳性率明显降低，说明 uc004coz.1 下调能够明显抑
3B）。Transwell 实验也显示 uc004coz.1 敲减后细胞 制细胞的增殖（P < 0.01，图4A、B）。

A B 2.0
siNC
si⁃uc004coz.1
1.5 1.5
Relative expression of uc004coz.1/β⁃actin 1.0 ** Cell viability 1.0 ** *

0.5
0.5
0 *
0
0 24 48 72
siNC si⁃uc004coz.1
Time（h）
C siNC si⁃uc004coz.1 D
200
Transwell cell 150 **

100
50

0
siNC si⁃uc004coz.1
A：The knock down efficiency of uc004coz.1；siNC represented the negative control group，and si⁃uc004coz.1 represented the siRNA knockdown
group. B：The growth trend of cells in siNC and si⁃uc004coz.1 was detected by CCK⁃8 at 0，24，48，and 72 h. C：The cell migration levels of siNC and
si⁃uc004coz.1 were detected by transwell assay（×100）. D：Statistical results of the number of migrating cells. Compared to the siNC group，P < 0.05，
*
**
P < 0.01（n=3）.
图3 uc004coz.1敲减效率与细胞增殖迁移检测
Figure 3 Detection of uc004coz.1 knock⁃down efficiency and cell proliferation and migration
A EdU Hoechst Merged B

siNC 0.6
EdU positive rate 0.4 **

0.2

si⁃uc004coz.1 0
siNC si⁃uc004coz.1

A：The cell proliferation was detected by EdU（×200）. Red represents proliferation⁃positive nuclear staining and blue represents nuclear hoechst
**
staining. B：Statistical results of the percentage of proliferative positive cells. Compared to the siNC group，P < 0.01（n=3）.
图4 EdU实验检测细胞增殖水平
Figure 4 Cell proliferation was detected by EdU