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第42卷第3期                           南京医科大学学报(自然科学版)
                  2022年3月                   Journal of Nanjing Medical University(Natural Sciences)     ·415 ·


               ·公共卫生与预防医学研究·

                南京市某地区大气多环芳烃污染及空气微生物多样性相关分析



                朱宇琪,吴 昊,热那古力·吾斯曼江,沈梦凡,谈依婷,李                         玥,王     丽,吴    倩 *
                南京医科大学公共卫生学院卫生检验与检疫学系,江苏                    南京 211166




               [摘   要] 目的:研究南京大气中多环芳烃污染水平,探究大气多环芳烃浓度与空气微生物群落的相关性。方法:采用气相色
                谱⁃质谱联用仪定量分析2019—2020年春秋季南京市大气中多环芳烃(polycyclic aromatic hydrocarbon,PAH)的浓度;同时采用
                16S rRNA测序测定空气中微生物群落,对大气PAH的浓度与微生物群落进行Pearson相关性分析。结果:通过分析2019年和
                2020年大气PAH浓度数据发现,大气中菲Phe、荧蒽Fla和芘Pyr占主要成分,2019年秋季大气PAH浓度明显高于春季。2019年
                大气总PAH浓度高于2020年。在微生物门水平上,厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)、变形菌门(Proteobacteria)
                均为2019年和2020年的优势菌门。在微生物属水平上,芽胞杆菌属为2019年与2020年共同优势菌属。同时,PAH污染与微
                球菌属(放线菌门)和芽胞杆菌属(厚壁菌门)的丰度呈正相关。结论:大气PAH污染可能与空气微生物的种类和丰度的变化
                有关,并具有潜在的健康风险。
               [关键词] 空气污染;多环芳烃;微生物;相关分析
               [中图分类号] R122.7                   [文献标志码] A                       [文章编号] 1007⁃4368(2022)03⁃415⁃06
                doi:10.7655/NYDXBNS20220318



                Studies on correlation of atmospheric PAH pollution and microbial diversity in a certain
                area of Nanjing

                ZHU Yuqi,WU Hao,RANAGUL Osman,SHEN Mengfan,TANG Yiting,LI Yue,WANG Li,WU Qian       *
                Department of Health Inspection and Quarantine,School of Public Health,Nanjing Medical University,Nanjing
                211166,China



               [Abstract] Objective:This study aims to observe the levels of atmospheric polycyclic aromatic hydrocarbon(PAH)in Nanjing,and
                explore the correlation between PAH and air microbial community. Methods:The concentration of PAH was determined by GC⁃MS,
                and microbial community diversity was analyzed by 16S rRNA gene sequencing. Pearson correlation analysis was conducted between
                the concentration of atmospheric PAH and the microbial community. Results:It was found that Phe,Fla and Pyr are the main
                components of atmospheric PAH. The total concentration of atmospheric PAH in 2019 was higher than that in 2020. The concentration
                of atmospheric PAH in autumn of 2019 was significantly higher than that in spring. At the level of phylum,the dominant phylum in
                both 2019 and 2020 are Firmicutes,Actinobacteria and Proteobacteria. In terms of genus,Bacillus is the common dominant bacteria of
                2019 and 2020. In the correlation analysis between PAH and microbial populations,it was found that the abundance of Bacillus in
                Firmicutes and Micrococcus in Actinobacteria were correlated with atmospheric PAH. Conclusion:Atmospheric PAH pollution may be
                related to the changes of species and abundance of air microorganisms which may be potential health risks.
               [Key words] air pollution;polycyclic aromatic hydrocarbons;microbial community;correlation analysis
                                                                              [J Nanjing Med Univ,2022,42(03):415⁃420]



                                                                      多 环 芳 烃(polycyclic aromatic hydrocarbon,
               [基金项目] 国家自然科学基金(82073630),国家自然基金
                                                                  PAH)是含有两个或两个以上苯环的碳氢化合物,主
                海外及港澳学者合作研究基金(81728018);江苏省自然科学
                                                                  要来源于化石燃料、木材等有机物的不完全燃烧,
                基金(BK20161571);江苏省高校自然科学研究重大项目
                                                                                                   [1]
               (16KJA330002)                                      广泛存在于大气、水、土壤等环境中 。可通过呼
                ∗                                                 吸、饮食、皮肤接触等多种途径进入人体,也可经胎
                通信作者(Corresponding author),E⁃mail:wuqian@njmu.edu.cn
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