Page 57 - 南京医科大学学报自然科学版

第42卷第6期 南京医科大学学报（自然科学版）
2022年6月 Journal of Nanjing Medical University（Natural Sciences） ·809 ·

·基础研究·

小鼠不同脂肪组织对冷应激的适应性反应

耿 蓉，罗 岩，孙 翠，向李倩，刘 煜 *
南京医科大学附属逸夫医院内分泌科，江苏 南京 211166

［摘 要］ 目的：明确C57BL/6J小鼠不同脂肪组织对于冷刺激适应性应答的相似点与差异。方法：建立冷刺激组及对照组小
鼠模型，测量体重、进食量等；HE染色观察各脂肪组织切片形态；荧光定量PCR法检测棕色化、线粒体功能等相关基因的表达
变化；Western blot检测产热等相关蛋白的表达情况；免疫组化检测脂肪组织交感神经支配；流式细胞术分析脂肪组织中巨噬
细胞的分型。结果：与米色和白色脂肪相比，棕色脂肪组织的产热相关基因解偶联蛋白1表达量最高，但冷刺激不能进一步促
进其表达；而冷刺激能够显著诱导米色脂肪组织中产热基因和蛋白的表达。冷刺激能够促进棕色脂肪和米色脂肪中交感神经
支配和巨噬细胞M2型极化。结论：冷刺激能够诱导米色脂肪组织棕色化，同时促进交感神经支配和巨噬细胞的M2型极化；
冷刺激对内脏白色脂肪组织的影响最小。
［关键词］ 冷刺激；脂肪组织；巨噬细胞；脂肪组织产热；脂肪组织炎症
［中图分类号］ R589.2 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）06⁃809⁃06
doi：10.7655/NYDXBNS20220607

The adaptive responses of different adipose tissues to cold stress in mice

GENG Rong，LUO Yan，SUN Cui，XIANG Liqian，LIU Yu *
Department of Endocrinology，Sir Run Run Hospital Affiliated to Nanjing Medical University，Nanjing 211166，China

［Abstract］ Objective：This study aims to clarify the similarities and differences of adaptive responses of different adipose tissues in
C57BL/6J mice to cold stress. Methods：Mice were divided into two groups，the control group which were housed under room
temperature individually and the cold stress group were housed under 6 ℃. Body weight，food intake and adipose tissue weight were
measured. Adipose tissue morphology were observed by HE staining. The genes related to browning，mitochondrial function were
quantified by qRT⁃PCR. The proteins related to thermogenesis were detected by Western blot. Adipose tissue sympathetic innervation
was shown by IHC staining. Macrophages polarization were tested by flow cytometry. Results：Thermogenic gene Ucp⁃1 expression
were most abundant in brown adipose tissues but could not be promoted by cold. By contrast，thermogenic gene expression could be
stimulated by cold stress in beige adipose tissue in both mRNA and protein levels. Simultaneously，sympathetic innervation and
macrophages M2 polarization could be induced in both brown and beige adipose tissue. Conclusion：Cold stress could promote
sympathetic innervation and macrophage M2 polarization in beige adipose tissue which could induced browning. And cold stimulation
has minimal role in regulating visceral white adipose tissues regarding themogenic gene expression and macrophage polarization.
［Key words］ cold stimulation；adipose tissue；macrophages；adipose tissue thermogenesis；adipose tissue inflammation
［J Nanjing Med Univ，2022，42（06）：809⁃814］

肥胖已成为全球公共卫生危机，中国是全球受 能量代谢平衡中重要的靶器官，不仅能够通过能量
肥胖影响人数最多的国家之一，约46%的成年人和 贮存和释放，还可以通过调节产热、改变局部炎症
15%的儿童肥胖或超重 ［1-2］ 。脂肪组织是调节机体 状态来参与机体能量代谢平衡 。冷刺激治疗肥胖
［3］
的潜在作用包括诱导脂肪组织适应性产热 、促进
［4］
［基金项目］ 国家自然科学基金（82070849）；江苏省“双创团
［5］
队”项目 2 型免疫、促进脂肪分解 等。
∗ 脂肪组织分为以下 3 种类型：棕色脂肪组织
通信作者（Corresponding author），E⁃mail：drliuyu@njmu.edu.cn