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第42卷第7期
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A iDC LPS⁃iDC tolDC LPS⁃tolDC
100 CFSE⁃ CFSE+ 100 CFSE⁃ CFSE+ 100 CFSE⁃ CFSE+ 100 CFSE⁃ CFSE+
63.0% 37.0% 86.8% 13.2% 43.3% 56.7% 54.8% 45.2%
80 80 80 80
2.5HIP组 60 60 60 60
40
40
40
40
20 20 20 20
0 0 0 0
10 0 10 1 10 2 10 3 10 4 10 0 10 1 10 2 10 3 10 4 10 0 10 1 10 2 10 3 10 4 10 0 10 1 10 2 10 3 10 4
100 CFSE⁃ CFSE+ 100 CFSE⁃ CFSE+ 100 CFSE⁃ CFSE+ 100 CFSE⁃ CFSE+
4.32% 95.7% 3.81% 96.2% 1.68% 98.3% 4.32% 95.7%
80 80 80 80
无关肽组 60 60 60 60
40
40
40
40
20 20 20 20
0 0 0 0
10 0 10 1 10 2 10 3 10 4 10 0 10 1 10 2 10 3 10 4 10 0 10 1 10 2 10 3 10 4 10 0 10 1 10 2 10 3 10 4
CFSE *** □ 无关肽组
100 *** ■ 2.5HIP组
( % ) 80
增殖比例 40
60
20
0
iDC LPS⁃iDC tolDC LPS⁃tolDC
B
iDC LPS⁃iDC tolDC LPS⁃tolDC
5 2.41% 83.1% 5 3.38% 86.6% 5 5
10 10 10 1.57% 17.1% 10 4.57% 45.9%
10 4 10 4 10 4 10 4
2.5HIP组 10 3 2 10 3 2 10 3 2 Q5 10 3 2
10
10
10
10
4.61
10 1 10 1 10 1 10 1
8.38% 6.12% 5.09% 4.96% 53.6% 27.8% 35.3% 14.2%
10 1 10 2 10 3 10 1 10 2 10 3 10 1 10 2 10 3 10 1 10 2 10 3
5 1.57% 6.22% 5 2.23% 6.91% 5 1.67% 2.75% 5 4.61% 4.41%
10 10 10 10
10 4 10 4 10 4 10 4
无关肽组 10 3 2 10 3 2 10 3 2 10 3 2
10
10
10
10
10 1 10 1 10 1 10 1
67.2% 25.0% 78.8% 12.1% 81.6% 14.0% 76.5% 14.5%
10 1 10 2 10 3 10 1 10 2 10 3 10 1 10 2 10 3 10 1 10 2 10 3
CD44 100 *** □ 无关肽组
( % ) 80 *** ■ 2.5HIP组
CD44 + CD69 + 60
40
20
0
iDC LPS⁃iDC tolDC
LPS⁃tolDC
A:BDC2.5 CD4 T细胞与各组2.5HIP⁃DC 按10∶1共培养后的增殖情况(图中灰色为CFSE 母峰对照组);B:活化情况(CD44 CD69 所占比
+
+
+
例)。两组比较, P < 0.001,n:3~4。
***
+
图3 BDC2.5 CD4 T细胞与负载2.5HIP的DC共培养后的增殖活化情况及产生Treg的比例
Figure 3 Proliferation,activation and proportion of Treg of BDC2.5 CD4 T cells co⁃cultured with 2.5HIP⁃loaded DC
+