Page 52 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第42卷第7期
·948 · Journal of Nanjing Medical University（Natural Sciences） 2022年7月

·基础研究·

温度/超声双重响应型相变液滴对水凝胶结构性能的调控

赵 婵，刘 昊，陈洁莹，徐雪琴，胡 克，段 磊 *
南京医科大学生物医学工程系，江苏 南京 211166

［摘 要］ 目的：制备温度/超声双重响应型脂质相变液滴（lipid phase⁃change droplet，LPCD），并将其与水凝胶进行复合，通过
温度或低强度聚焦超声（low intensity focused ultrasound，LIFU）调控LPCD的内核发生液⁃气相转变，继而调控水凝胶的结构和
力学性能。方法：首先通过薄膜水化法制备出脂质膜壳的全氟己烷（perfluorohexane，PFH）相变液滴，对其在温度和LIFU调控
下的形貌变化、相转变能力进行表征，同时评估液⁃气相变前后LPCD的超声造影能力。在此基础上将其与水凝胶进行复合，通
过各项表征评估温度/超声引起的液滴相变对水凝胶内部结构、浮力和机械性能的影响。结果：LPCD在温度或超声调控下有
良好的相转变能力和超声造影能力。脂质液滴相变可使水凝胶内部生成直径在微米量级的诸多气孔，浮力得到显著提高，
压缩应力和溶胀性能也有所变化。结论：脂质相变液滴⁃水凝胶复合材料（LPCD@Hy）是一种有潜力的智能响应型诊疗用微
纳材料。
［关键词］ 脂质相变液滴；水凝胶；造影剂；超声成像
［中图分类号］ R318.5 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）07⁃948⁃09
doi：10.7655/NYDXBNS20220706

Structure and properties regulation of hydrogels by temperature/ultrasound dual⁃responsive

phase⁃change droplets

ZHAO Chan，LIU Hao，CHEN Jieying，XUE Xueqin，HU Ke，DUAN Lei *
Department of Biomedical Engineering，Nanjing Medical University，Nanjing 211166，China

［Abstract］ Objective：Prepare temperature/ultrasound dual⁃responsive lipid phase⁃change droplet（LPCD）and combine them with
hydrogels to regulate liquid⁃gas transitions in the core of lipid droplets by temperature or low intensity focused ultrasound（LIFU），and
then regulate the structure and mechanical properties of the hydrogels. Methods：Firstly，perfluorohexane（PFH）phase ⁃ change
droplets of lipid membrane shell were prepared by thin⁃film hydration method. The morphological changes and phase transition ability
under the control of temperature and LIFU were characterized. At the same time，the contrast⁃enhanced ability of LPCD before and
after liquid⁃gas phase transition was evaluated. On this basis，it was combined with hydrogels，and the effects of temperature/ultrasound
induced droplet transition on the internal structure，buoyancy and mechanical properties of hydrogels were evaluated by
characterization. Results：LPCD had good phase transition ability and contrast ⁃ enhanced ultrasound ability under the control of
temperature or ultrasound. Lipid droplet phase transitions caused many micropores inside the hydrogel，which significantly improved
buoyancy，varying compressive stress and swelling properties. Conclusion：Lipid phase⁃change droplets⁃hydrogel composite material
（LPCD@Hy）is a kind of potential intelligent response micro/nano material for diagnosis and treatment.
［Key words］ lipid phase⁃change droplet；hydrogel；contrast agent；ultrasound imaging
［J Nanjing Med Univ，2022，42（07）：948⁃956］

相变液滴（phase⁃change droplet，PCD）是一种在
［基金项目］ 国家重点研发计划（2019YFA0210104）；国家
微气泡研究基础上发展起来的具有液⁃气相变能力
自然科学基金（81701833）；江苏省重点大学生创新创业训练
［1］
计划项目（KY124J2019065） 的诊疗一体化微纳材料 ，相变液滴使用具有低溶
∗ 解度和扩散率的全氟化碳（perfluorocarbon，PFC）作
通信作者（Corresponding author），E⁃mail：duanlei@njmu.edu.cn