Page 61 - 南京医科大学学报自然科学版

第42卷第7期 南京医科大学学报（自然科学版）
2022年7月 Journal of Nanjing Medical University（Natural Sciences） ·957 ·

·基础研究·

负载抗菌多肽温敏水凝胶的制备及性能研究

吴 迪，王 萍，雷 晨，吴 维，马珊珊，汤春波 *
南京医科大学附属口腔医院口腔种植科，江苏省口腔疾病研究重点实验室，江苏省口腔转化医学工程研究中心，江苏 南京

210029

［摘 要］ 目的：构建透明质酸（hyaluronic acid，HA）/羟丙基甲基纤维素（hydroxypropyl methylcellulose，HPMC）/甘油温敏水凝
胶体系并搭载抗菌多肽芬母多肽（Funme peptide，FP），检测水凝胶的相关理化性质、抗菌性能以及对小鼠皮肤创伤愈合的促进作
用。方法：采用物理交联的方法制备HA/HPMC/甘油⁃FP温敏水凝胶，分析HA和甘油的含量对凝胶化温度（gelation temperature，
Tgel ）的影响。利用流变学检测、傅里叶变换红外光谱仪以及扫描电子显微镜对制备的水凝胶进行理化性能表征。同时对水凝
胶的生物相容性和抗菌性进行观察研究。构建小鼠背部直径10 mm的全层皮肤缺损感染创面模型，在注射水凝胶后，于第5、
10、15天观察创面愈合情况，计算创面愈合率。结果：在HA、HPMC和甘油的比例为2∶7∶20时，水凝胶具有理想的Tgel；水凝胶
内部呈孔隙率高、孔径较均匀的三维结构，各成分间未发生化学反应，同时该水凝胶具有良好的生物相容性，对大肠杆菌
（Escherichia coli，E. coli）具有显著的抗菌效果。动物实验表明，HA/HPMC/甘油⁃FP水凝胶处理的小鼠背部创面愈合明显优于
对照组。结论：该温敏水凝胶在医用创伤敷料领域具有良好的应用前景。
［关键词］ 温敏水凝胶；羟丙基甲基纤维素；透明质酸；抗菌多肽；抗菌性能；创伤愈合
［中图分类号］ R783.1 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）07⁃957⁃08
doi：10.7655/NYDXBNS20220707

Preparation and properties of antimicrobial peptide⁃loaded thermosensitive hydrogel

WU Di，WANG Ping，LEI Chen，WU Wei，MA Shanshan，TANG Chunbo *
Department of Dental Implant and Prosthodontics，the Affiliated Stomatological Hospital of Nanjing Medical
University，Jiangsu Province Key Laboratory of Oral Diseases，Jiangsu Province Engineering Research Center of
Stomatological Translational Medicine，Nanjing 210029，China

［Abstract］ Objective：Preparation and characterization of a thermo⁃sensitive hydrogel consisting of hyaluronic acid（HA），hydroxypropyl
methylcellulose（HPMC）and glycerol，and loaded with antimicrobial peptide Funme（FP）. Methods：HA/HPMC/Glycerol⁃FP hydrogels
were synthesized by physical cross⁃linking，and the thermosensitive behavior of the hydrogels was analyzed. The physicochemical
properties of the hydrogels were characterized by rheological test，Fourier transform infrared spectroscopy（FTIR）and scanning
electron microscope（SEM）. The biocompatibility and antibacterial activities were also investigated in vitro. A full⁃thickness skin defect
model with a diameter of 10 mm was constructed on the back of the mouse and the wound healing rate was recorded after treatment with
hydrogel. Results：HA/HPMC/Glycerol⁃FP hydrogel exhibited desirable gel temperature when the ratio of HA，HPMC and glycerol was
2∶7∶20 and no chemical reactions occurred during hydrogelation. Moreover，the hydrogel exhibited good biocompatibility on L929
cells and showed a significant inhibitory effect on Escherichia coli（E. coli）. Animal experiments showed that wound healing in the HA/
HPMC/Glycerol⁃FP group was significantly better than the control group. Conclusion：HA/HPMC/Glycerol⁃FP is a conductive material
that has great application prospects in bio⁃medical dressings.
［Key words］ thermo⁃sensitive hydrogel；hydroxypropyl methylcellulose；hyaluronic acid；antimicrobial peptide；antibacterial activi⁃
ty；wound healing
［J Nanjing Med Univ，2022，42（07）：957⁃964］

［基金项目］ 国家自然科学基金（82170993）；东南大学⁃南京医科大学合作研究项目（2242018K3DN03）；政府间国际科技创新
合作重点专项（2018YFE0194100）
∗
通信作者（Corresponding author），E⁃mail：cbtang@njmu.edu.cn