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第43卷第12期 李靖燕,曹林霞,马萌萌,等. 支气管肺发育不良模型小鼠青春期肺部菌群特点[J].
2023年12月 南京医科大学学报(自然科学版),2023,43(12):1643-1649 ·1647 ·
A g_Actinobacteriota g_Bacteroidota
g_Proteobacteria g_Chlorofiexi B 100 g_Ralstonia
g_Firmicutes g_Deinococcota g_Rhodococcus g_Lactobacillus
g_unidentified Other g_Acinetobacter
g_unidentified g_uncultured
100 g_Burkholderia⁃Caballeronia⁃
80 g_Aeromonas
g_Pseudomonas Paraburkholderia
g_Castellaniella
80 ( % ) g_Staphylococcus g_Sphingomonas
g_Vibrionimonas
( % ) 60 60 g_Brochothrix g_Myroides
g_Serratia
g_Aquabacteriur
g_Streptococcus
g_Pelomonas
相对丰富 40 相对丰富 40 g_Methylobacterium⁃Methylorubrum
g_Lactococcus
g_Corynebacterium
g_Rhodopseudomonas
20 20 g_Massilia
g_Cloacibacterium
g_Rhodobacter
0 0 g_Truepera
Ⅰa组 Ⅰb组 Ⅱa组 Ⅱb组 Ⅰa组 Ⅰb组 Ⅱa组 Ⅱb组 Other
A:门水平图注(Ⅰa:空气雌性组;Ⅰb:空气雄性组;Ⅱa:高氧雌性组;Ⅱb:高氧雄性组);B:属水平。
图4 小鼠肺部菌群在门、属水平物种组成及相对丰度
Figure 4 The relative abundance of pulmonary microbiome inmice at the phylum and genus level
A C
95% CI 95% CI
空气组 高氧组 空气组 高氧组
g_Aeromonas 0.028
g_Proteobacteria 0.013
g_Acinetobacter 0.036
g_Vibrionomonas 0.535
g_Bacteroidota 0.914
g_Brochothrix 0.928
g_unldentified 0.188 P值 g_Ralstonla 0.433
g_Pseudomonas 0.914 P值
g_Firmicutes 0.164 g_Aquabacterium 0.148
g_Lactobacillus 0.136
g_Actinobacteriota 0.178 g_unldentified 0.213
0.188
g_Staphylococcus
0 20 40 60 -30 0 30 60 0.262
Mean proportion(%) Difference in mean proportion(%) g_Rhodococcus 0.189
0 10 20 30 40 -50 -25 0 25
Mean proportion(%) Difference in mean proportion(%)
B D
95% CI 95% CI
Ⅰb组 Ⅱb组 Ⅰb组 Ⅱb组
g_Aeromonas 0.040
g_Proteobacteria 7.37e⁃05 g_Acinetobacter 0.027
g_Pseudomonas 0.35
g_unldentified 0.295 g_Brochothrix 0.378
P值 g_Pelomonas 0.040 P值
g_Firmicutes 0.445 g_Aquabacterium 0.036
g_unldentified 0.295
g_Staphylococcus 0.368
g_Actinobacteriota 0.030
g_Rhodococcus 0.032
0 20 40 60 80 -50 0 50 0 20 40 -80 -40 0 40
Mean proportion(%) Difference in mean proportion(%) Mean proportion(%) Difference in mean proportion(%)
A、B:门水平;C、D:属水平。Ⅰb:空气雄性组;Ⅱb:高氧雄性组
图5 小鼠肺部菌群在门、属水平的差异分析
Figure 5 Difference analysis of pulmonary flora in mice at phylum and genus level
Ⅰa Ⅰb Ⅱb
s_Aeromonas_caviae
o_Chitinophagales
g_Corynebacterium
g_Clostridium_sensu_stricto_1
o_Clostridiales
f_Clostridiaceae
s_Staphylococcus_nepalensis
s_bacterium
g_leotgalicoccus
s_leotgalicoccus_aeroatus
0 1 2 3 4 5
lg(LDA SCORE)
Ⅰa空气雌性组;Ⅰb空气雄性组;Ⅱb高氧雄性组。
图 6 小鼠肺部菌群 LEfSe 分析柱状图
Figure 6 Histogram of LEfSe analysis of pulmonary microbiome in mice