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表1 利用单细胞测序获得的与妇科恶性肿瘤相关的关键发现
Table 1 Key findings relating to gynecological malignancies obtained using single⁃cell sequencing

Tumor Technology Key findings
CC scRNA⁃seq，ST⁃seq Produced a spatial map illustrating the four developmental stages of HPV⁃related
［20］
CC and identified three distinct‘HPV⁃related epithelial clusters’
scRNA⁃seq Revealed dynamic changes in the TME during the progression of CC，the primary
tumor exhibited an immune ⁃ suppressive state，whereas an immune response in
metastatic lymph nodes was activated ［34］
scRNA⁃seq Revealed that squamous and columnar epithelia originate from distinct lineage ⁃
specific populations of ectocervical resident stem cells;These stem cell populations
were regulated by opposing Wnt signals from the stroma，and disruption of Wnt sig⁃
［21］
naling led to carcinogenesis
OC scRNA⁃seq，bulk⁃seq Identified seven subtypes within the primary tumor，and among them，the CYR61 +
+
subtype was determined to be associated with recurrence，while the RGS5 CAF
subtype promoted tumor metastasis；CYR61 and RGS5 could serve as predictive
factors for recurrent EOC ［23］
scCOOL⁃seq Revealed mechanisms associated with the upregulation of genes involved in IFN
signaling transduction，metallothioneins，and metabolism in OC；established new
［25］
potential therapeutic targets for the treatment of OC
scRNA⁃seq Revealed the cellular landscape of HGSOC and elucidated the mechanisms by
which mCAFs enhance the invasive capabilities of ovarian tumor cells;detected
TIGIT as a potential immunotherapeutic molecular target for HGSOC ［35］
scRNA⁃seq Elucidated the distinct functions exercised by different cell types within the HG⁃
SOC ascites ecosystem. The communication and interaction among these different
cells may play a balancing role in disease progression and response to treatment.
Consequently，altering this balance therapeutically could be a therapeutic pathway
to reshaping the drug⁃resistant environment ［38］
scRNA⁃seq，TCR⁃seq Depicted the single ⁃ cell landscape of five sites associated with OC，including
omentum metastasis and malignant ascites;revealed the interconnections between
［39］
the malignant ascites ecosystem and tumor sites
EC scRNA⁃seq Identified a characteristic subpopulation associated with the development of EEC，
which is rarely expressed in normal endometrial tissue，but shows an increasing ex⁃
pression pattern in AEH and EEC ［31］
scRNA⁃seq Revealed ciliated cell markers DYDC2，CTH，FOXJ1，and p73 as well as the se⁃
cretory cell marker MPST，which can be utilized to improve clinical risk stratifica⁃
［32］
tion for EC patients
scRNA⁃seq Detected the presence of the mature tertiary lymphoid structure marker L1CAM，
which serves as a TLS immunohistochemical marker，improving the accuracy of
predicting recurrence and prognosis in EC patients ［36］
scDNA⁃seq，scRNA⁃seq， Identified molecular biomarkers such as PIK3R1 and CTNNB1 which contribute
single⁃cell proteomics to the investigation of drug resistance mechanisms in EC and guide stratified man⁃
［40］
agement and precision treatment research for EC patients

［参考文献］ CA Cancer J Clin，2021，71（3）：209-249
［2］ ZHENG R，ZHANG S，ZENG H，et al. Cancer incidence
［1］ SUNG H，FERLAY J，SIEGEL R L，et al. Global cancer
and mortality in China，2016［J］. J Natl Cancer Cent，
statistics 2020：GLOBOCAN estimates of incidence and
2022，2（1）：1-9
mortality worldwide for 36 cancers in 185 countries［J］. ［3］ ALFANO C M，LEACH C R，SMITH T G，et al. Equitably