Page 69 - 南京医科大学自然版

第44卷第6期 马晴晴，顾圣玮，王红玉，等. 基于线粒体自噬相关基因的哮喘亚型鉴定及预测模型构建研究［J］.
2024年6月 南京医科大学学报（自然科学版），2024，44（6）：802-811 ·805 ·

A 2 B
Group GSE76226 & GSE63142 & GSE67472
SRC Asthma group
CSNK2B 1 Normal group ***
MAP1LC3A
ULK1 5.1
MFN1 0
CSNK2A2
PINK1 4.8
-1 GSVA score of MRG geneset
UBB
UBC
PGAM5 -2 4.5
SQSTM1
ATG5 4.2
MAP1LC3B
MFN2 Normal group Asthma group
TOMM5
FUNDC1
RPS27A
ATG12
CSNK2A1
TOMM20
UBA52
TOMM22
C D
GSE179156
TOMM5
** ｜ 6 FUNDC1 -lg（P value）
GSVA score of MRG geneset 9.0 value） （P ｜lg 4 2 SQSTM1 UBA52 PGAM5 6 4 2
9.3
TOMM22

MFN2
UBB
8.7
8.4 0
Normal group Asthma group -0.50 -0.25 0 0.25 0.50
Log2FC
A：The heatmap of the expression pattern of 22 MRGs. B：The boxplot of the overall difference of MRGs expression between healthy individuals and
asthma patients. C：Confirmation of the overall difference in MRGs expression between healthy individuals and asthma patients in the dataset
GSE179156. D：The volcano map including 8 MRGs that differentially expressed in asthma，in which negative log 2FC represents downregulation and posi⁃
**
tive log2FC represents upregulation of the gene. P < 0.01 and *** P < 0.001.
图1 哮喘气道MRG的差异表达
Figure 1 Differential expression of MRG in asthmatic airway

A Control Asthma 8 ***
expression 6

×200 （mean intensity） 4
TOMM5 2

0
Control Asthma
B
Control IL⁃13
5 4 ***
TOMM5 expression （mean intensity） 3
×400 2 1

0
Control IL⁃13
A：Immunohistochemical staining of lung tissues obtained from OVA⁃induced acute asthma mice（n=6）. B：Immunocytochemical staining of primary
epithelial cultures obtained from the murine airway（n=5）. *** P < 0.001.
图2 免疫组化检测哮喘气道的TOMM5蛋白表达
Figure 2 TOMM5 expression in asthmatic airway detected by immunohistochemistry