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A -/- +/- B -/- +/-
Rictor Rictor Rictor Rictor
10 6 10 6
Q2⁃UL Q2⁃UR Q2⁃UL Q2⁃UR
（0.02%） （20.45%） （0.01%） （20.04%）
10 5 10 5
M1 M1 PB450⁃A 10 4 PB450⁃A 10 4

Q2⁃LL Q2⁃LR Q2⁃LL Q2⁃LR
0 0
（1.53%） （77.90%） （0.35%） （70.00%）
0 10 2 10 4 10 5 10 6 0 10 2 10 4 10 5 10 6
PE⁃A PE⁃A
M1+TFDS 10 6 5 （0.02%） （15.32%） 10 6 5 （0.01%） （22.07%）
Q2⁃UL
Q2⁃UR
Q2⁃UR
Q2⁃UL
M1+TFDS PB450⁃A 10 4 PB450⁃A 10 4
10
10
Q2⁃LL Q2⁃LR Q2⁃LL Q2⁃LR
0 0
（5.56%） （79.11%） （0.01%） （77.12%）
0 10 2 10 4 10 5 10 6 0 10 2 10 4 10 5 10 6
PE⁃A PE⁃A
C D
40 *** 5 ***
（%） 30 *** 4
F4/80 + iNOS + macrophages 20 Relative expression of iNOS 2 mRNA 3 *

10
0 1 0
+M1 +M1 +M1 +M1
-/- +M1+TFDS +/- +M1+TFDS -/- +M1+TFDS +/- +M1+TFDS
Rictor -/- Rictor +/- Rictor -/- Rictor +/-
Rictor Rictor Rictor Rictor

A：Cellular immunofluorescence results after TFDS intervention on M1 macrophages. B，C：Flow cytometry（B）and semi quantitative results（C）of
M1 macrophages after TFDS intervention. D：Semi quantitative results of intracellular iNOS mRNA levels after TFDS intervention in M1 macrophages.
* ***
P < 0.05， P < 0.001（n=5）.
图3 TFDS对体外M1型巨噬细胞极化的影响
Figure 3 The effect of TFDS on polarization of M1 macrophages in vitro

中的作用，更为 TFDS 广泛应用于泌尿系结石的防 ZHANG Wei participated in experimental design；and LU Pei
治提供了理论依据。 was responsible for experimental design as well as reviewing
利益冲突声明： and revising the article.
所有作者声明无利益冲突。 ［参考文献］
Conflict of Interests：
［1］ JIANG Q，DONG C，HE Z，et al. Research landscape and
All authors declare no conflicts of interests.
pharmacological mechanisms of traditional Chinese medi⁃
作者贡献声明：
cines in treating and preventing urolithiasis：unearthing
宗益平负责实验设计及操作、撰写文章初稿；张俊麒负
an anti ⁃ urolithic treasure trove［J］. J Ethnopharmacol.
责构建动物模型；王子杰负责实验设计及操作；韩鹏参与实
2024，11（334）：118502
验操作；张炜参与实验设计；鲁佩负责实验设计和文章审阅
［2］ SMEULDERS N，CHO A，ALSHAIBAN A，et al. Shock⁃
修改。
waves and the rolling stones：an overview of pediatric
Author’s Contributions：
stone disease［J］. Kidney Int Rep，2023，8（2）：215-228
ZONG Yiping was responsible for experimental design and
［3］ KACHKOUL R，TOUIMI G B，EL MOUHRI G，et al. Uro⁃
operation，as well as writing the initial draft of the article； lithiasis：history，epidemiology，aetiologic factors and man⁃
ZHANG Junqi was responsible for constructing the animal mod⁃ agement［J］. Malays J Pathol，2023，45（3）：333-352
el；WANG Zijie was responsible for experimental design and op⁃ ［4］ ZOU X C，LUO C W，YUAN R M，et al. Develop a ra⁃
eration；HAN Peng participated in experimental operations； diomics ⁃ based machine learning model to predict the